Igori Davaajargal, Lim Seungmo, Kim Se Eun, Shin Ah Young, Kwon Suk-Yoon, Moon Jae Sun
Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia.
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Arch Virol. 2022 Dec;167(12):2767-2770. doi: 10.1007/s00705-022-05571-y. Epub 2022 Aug 31.
The complete genomic nucleotide sequence of hemisteptia virus A (HemVA) from a Hemisteptia lyrata Bunge plant in South Korea was identified by high-throughput sequencing. The HemVA genome consists of 6,122 nucleotides and contains seven putative open reading frames, ORF0-5 and ORF3a, encoding the putative proteins P0-P5 and P3a, respectively. Pairwise amino acid sequence analysis shows that the HemVA P1-P5 proteins have the highest sequence identity (23.68%-54.15%) to the corresponding proteins of members of the families Solemoviridae and Tombusviridae. Phylogenetic analysis of the P1-P2 and P3 amino acid sequences indicated that HemVA should be classified as a member of a distinct species in the genus Polerovirus.
通过高通量测序鉴定了来自韩国泥胡菜植株的泥胡菜病毒A（HemVA）的完整基因组核苷酸序列。HemVA基因组由6122个核苷酸组成，包含7个推定的开放阅读框，即ORF0 - 5和ORF3a，分别编码推定的蛋白质P0 - P5和P3a。氨基酸序列比对分析表明，HemVA的P1 - P5蛋白与独脚金病毒科和番茄丛矮病毒科成员的相应蛋白具有最高的序列同一性（23.68% - 54.15%）。对P1 - P2和P3氨基酸序列的系统发育分析表明，HemVA应被归类为马铃薯卷叶病毒属中一个独特物种的成员。
