全甲基化寡糖的连续流快原子轰击质谱法：填充毛细管柱液相色谱-快原子轰击质谱法直接混合物分析的比较研究
Continuous-flow fast atom bombardment-mass spectrometry of permethylated oligosaccharides: a comparative study of direct mixture analysis with packed capillary column liquid chromatography-fast atom bombardment-mass spectrometry.
作者信息
Boulenguer P, Leroy Y, Alonso J M, Montreuil J, Ricart G, Colbert C, Duquet D, Dewaele C, Fournet B
机构信息
Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres Artois (UA du CNRS No. 217), Villeneuve d'Ascq, France.
出版信息
Anal Biochem. 1988 Jan;168(1):164-70. doi: 10.1016/0003-2697(88)90024-3.
Conventional positive fast atom bombardment (FAB) and continuous-flow FAB analysis were carried out with permethylated lacto-N-tetraose. This latter method, a new approach, has been used to analyze a mixture of permethylated oligosaccharides by liquid chromatography-mass spectrometry (LC-MS) with a packed capillary fused-silica column and the continuous-flow probe as interface. Under these conditions, we found the continuous-flow probe to be superior to the conventional probe because its low matrix level increased the signal-to-noise ratio. The analysis of the mixture of permethylated oligosaccharide alditols obtained from hen ovomucoid by LC-MS using the continuous-flow probe as interface is described.
采用全甲基化的乳糖 - N - 四糖进行常规正离子快原子轰击（FAB）和连续流FAB分析。后一种方法是一种新方法，已用于通过液相色谱 - 质谱联用（LC - MS），使用填充毛细管熔融石英柱和连续流探头作为接口来分析全甲基化寡糖混合物。在这些条件下，我们发现连续流探头优于传统探头，因为其低基质水平提高了信噪比。本文描述了使用连续流探头作为接口，通过LC - MS对从鸡卵类粘蛋白获得的全甲基化寡糖糖醇混合物进行的分析。
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