CNR-Institute for the Electromagnetic Sensing of the Environment, Via Diocleziano 328, 80124, Naples, Italy.
CNR-Institute for Biostructures and Bioimaging, Via Mezzocannone, 16, 80134, Naples, Italy.
Environ Res. 2021 May;196:110935. doi: 10.1016/j.envres.2021.110935. Epub 2021 Feb 26.
In previous studies we demonstrated that radiofrequency (RF) electromagnetic fields (EMF) is able to reduce DNA damage induced by a subsequent treatment with genotoxic agents, resembling the adaptive response, a phenomenon well known in radiobiology. In this study we report on the capability of the culture medium from SH-SY5Y neuroblastoma cells exposed to 1950 MHz to elicit, in recipient non-exposed cells, a reduction of menadione-induced DNA damage (P < 0.05; comet assay), indicating the capability of non-ionizing radiation to elicit a bystander effect. A comparable reduction was also detected in cultures directly exposed to the same EMF conditions (P < 0.05), confirming the adaptive response. In the same exposure conditions, we also evidenced an increase of heat shock protein 70 (hsp70) in culture medium of cells exposed to RF with respect to sham exposed ones (P < 0.05; western blot analysis), while no differences were detected in the intracellular content of hsp70. On the whole, our results evidence a protective effect of RF against menadione-induced DNA damage in directly and non-directly exposed cells, and suggest hsp70 pathway to be investigated as one of the potential candidate underpinning the interaction between RF exposure and biological systems.
在之前的研究中，我们证明了射频（RF）电磁场（EMF）能够减少随后与遗传毒性剂处理引起的 DNA 损伤，类似于适应性反应，这是放射生物学中众所周知的现象。在这项研究中，我们报告了暴露于 1950 MHz 的 SH-SY5Y 神经母细胞瘤细胞的培养基在未暴露的受体细胞中引发降低甲萘醌诱导的 DNA 损伤的能力（P <0.05；彗星试验），表明非电离辐射有能力引发旁观者效应。在直接暴露于相同 EMF 条件的培养物中也检测到类似的降低（P <0.05），证实了适应性反应。在相同的暴露条件下，与假暴露的细胞相比，我们还在暴露于 RF 的细胞的培养基中检测到热休克蛋白 70（hsp70）的增加（P <0.05；western blot 分析），而细胞内 hsp70 的含量没有差异。总的来说，我们的结果证明了 RF 对直接和非直接暴露细胞中甲萘醌诱导的 DNA 损伤具有保护作用，并表明 hsp70 途径可作为潜在候选途径之一，以支持 RF 暴露与生物系统之间的相互作用。
