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评价射频电磁场对体外伤口愈合模型中细胞迁移和细胞因子表达变化的影响。

Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model.

机构信息

Department of Medicine and Science of Aging, University "G. d'Annunzio", Via dei Vestini, 66100 Chieti, Italy.

Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio", Via dei Vestini, 66100 Chieti, Italy.

出版信息

Int J Mol Sci. 2022 Feb 17;23(4):2205. doi: 10.3390/ijms23042205.

DOI:10.3390/ijms23042205
PMID:35216321
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8879593/
Abstract

Wound healing (WH) proceeds through four distinct phases: hemostasis, inflammation, proliferation, and remodeling. Impaired WH may be the consequence of the alteration of one of these phases and represents a significant health and economic burden to millions of individuals. Thus, new therapeutic strategies are the topics of intense research worldwide. Although radiofrequency electromagnetic field (RF-EMF) has many medical applications in rehabilitation, pain associated with musculoskeletal disorders, and degenerative joint disorders, its impact on WH is not fully understood. The process of WH begins just after injury and continues during the inflammatory and proliferative phases. A thorough understanding of the mechanisms by which RF-EMF can improve WH is required before it can be used as a non-invasive, inexpensive, and easily self-applicable therapeutic strategy. Thus, the aim of this study is to explore the therapeutic potential of different exposure setups of RF-EMF to drive faster healing, evaluating the keratinocytes migration, cytokines, and matrix metalloproteinases (MMPs) expression. The results showed that RF-EMF treatment promotes keratinocytes' migration and regulates the expression of genes involved in healing, such as MMPs, tissue inhibitors of metalloproteinases, and pro/anti-inflammatory cytokines, to improve WH.

摘要

伤口愈合（WH）经历四个不同的阶段：止血、炎症、增殖和重塑。其中任何一个阶段的改变都可能导致 WH 受损，这对数百万个人的健康和经济造成了重大负担。因此，新的治疗策略是全球研究的热点。尽管射频电磁场（RF-EMF）在康复、与肌肉骨骼疾病相关的疼痛和退行性关节疾病中有许多医疗应用，但它对 WH 的影响尚未完全了解。WH 的过程在受伤后立即开始，并在炎症和增殖阶段持续进行。在将 RF-EMF 用作非侵入性、廉价且易于自我应用的治疗策略之前，需要充分了解 RF-EMF 改善 WH 的机制。因此，本研究旨在探索不同 RF-EMF 暴露设置的治疗潜力，以促进更快的愈合，评估角质形成细胞的迁移、细胞因子和基质金属蛋白酶（MMPs）的表达。结果表明，RF-EMF 治疗可促进角质形成细胞的迁移，并调节与愈合相关的基因表达，如 MMPs、金属蛋白酶组织抑制剂和促炎/抗炎细胞因子，从而改善 WH。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfa7/8879593/180f53aa3781/ijms-23-02205-g007.jpg
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