Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal, India.
Department of General and Inorganic Chemistry, Institute of Chemistry, Saratov State University, Saratov, Russia.
Drug Discov Today. 2021 Jun;26(6):1501-1509. doi: 10.1016/j.drudis.2021.02.023. Epub 2021 Feb 26.
Circulating cell-free miRNAs (ccf-miRs) have gained significant interest as biomarkers for lung cancer (LC) diagnosis. However, the clinical application of ccf-miRs is mainly limited by time, cost, and expertise-related problems of existing detection strategies. Recently, the development of different point-of-care (POC) approaches offers useful on-site platforms, because these technologies have important features such as portability, rapid turnaround time, minimal sample requirement, and cost-effectiveness. In this review, we discuss different POC approaches for detecting ccf-miRs and highlight the utility of incorporating nanomaterials for enhanced biorecognition and signal transduction, further improving their diagnostic applicability in LC settings.
循环无细胞 miRNA（ccf-miRs）作为肺癌（LC）诊断的生物标志物引起了广泛关注。然而，ccf-miRs 的临床应用主要受到现有检测策略在时间、成本和专业知识方面问题的限制。最近，不同即时检测（POC）方法的发展提供了有用的现场检测平台，因为这些技术具有便携性、快速周转时间、最小样本需求和成本效益等重要特点。在本综述中，我们讨论了用于检测 ccf-miRs 的不同 POC 方法，并强调了将纳米材料纳入其中以增强生物识别和信号转导的效用，进一步提高了它们在 LC 环境中的诊断适用性。
