Shandilya Ruchita, Bhargava Arpit, Ratre Pooja, Kumari Roshani, Tiwari Rajnarayan, Chauhan Prachi, Mishra Pradyumna Kumar
Department of Molecular Biology, ICMR-National Institute for Research in Environmental Health, Bhopal 462030, India.
ACS Omega. 2022 Jul 22;7(30):26601-26609. doi: 10.1021/acsomega.2c02802. eCollection 2022 Aug 2.
Recent progress in the field of nanophotonics has opened up novel avenues for developing nanomaterial-based biosensing systems, which can detect various disease-specific biomarkers, including long noncoding RNAs (lncRNAs) known to circulate in biological fluids. Herein, we designed and developed a nanophotonic approach for rapid and specific capture of lncRNAs using oligonucleotide-conjugated graphene quantum-dot-nanoconjugates. The method offers accurate identification of the target lncRNAs with high selectivity, despite the presence of other molecules in the given sample. The observations also pointed toward the high feasibility and simplicity of the method in the selective determination of lncRNAs. Overall, the approach has the potential of assessing lncRNA expression as a function of disease initiation and progression.
纳米光子学领域的最新进展为开发基于纳米材料的生物传感系统开辟了新途径，这种系统可以检测各种疾病特异性生物标志物，包括已知在生物体液中循环的长链非编码RNA（lncRNA）。在此，我们设计并开发了一种纳米光子学方法，用于使用寡核苷酸共轭石墨烯量子点纳米共轭物快速、特异性地捕获lncRNA。该方法能够在给定样品中存在其他分子的情况下，以高选择性准确鉴定目标lncRNA。这些观察结果还表明该方法在选择性测定lncRNA方面具有高度的可行性和简便性。总体而言，该方法具有评估lncRNA表达作为疾病发生和发展函数的潜力。
