Department of Animal Science and Technology, National Taiwan University, Taipei City 106, Taiwan; Poultry Industry Section, Department of Animal Industry, Council of Agriculture, Executive Yuan, Taipei City 100, Taiwan.
Department of Animal Science and Technology, National Taiwan University, Taipei City 106, Taiwan.
Poult Sci. 2021 Apr;100(4):101009. doi: 10.1016/j.psj.2021.01.031. Epub 2021 Jan 20.
Approximately 400 metric tons of egg chalazae, a byproduct in the liquid-egg processing plant, are produced yearly but always regarded as a waste in Taiwan. Our team successfully developed a crude egg chalaza hydrolysate by protease-A digestion (CCH-A). Free branched-chain amino acids, 3-aminoisobutyric acid, and β-alanine, and anserine were assayed in the CCH-A used in this study. Besides, the in vitro bile-acid binding ability and inhibitory lipase activity of CCH-As were demonstrated. Then, high-fat diet feeding for 10 wk caused hyperlipidemia, insulin resistance, and hepatosteatosis in hamsters (P < 0.05). However, CCH-A co-treatment decreased serum/liver triglyceride levels and lipid accumulation in livers by increasing daily fecal lipid/bile-acid outputs, upregulating fatty-acid β oxidation, and downregulating fatty-acid biosynthesis in livers (P < 0.05). CCH-A co-treatment also amended insulin resistance, augmented hepatic antioxidant capacity, and decreased liver damages and inflammatory responses (P < 0.05). Taken together, our results do not only demonstrate the hepatoprotective effects of CCH-As against a chronic high-fat dietary habit, achieving effects similar to Simvastatin, but also decrease the environmental burden of handling chalazae in the liquid-egg industry.
每年约有 400 公吨的鸡蛋卵黄系带，这是液体蛋加工厂的副产物，但在台湾一直被视为废物。我们的团队成功地通过蛋白酶-A 消化开发了一种粗制的鸡蛋卵黄系带水解物（CCH-A）。本研究中使用的 CCH-A 中检测到游离支链氨基酸、3-氨基异丁酸和β-丙氨酸以及鹅肌肽。此外，还证明了 CCH-As 的体外胆汁酸结合能力和抑制脂肪酶活性。然后，高脂饮食喂养 10 周会导致仓鼠发生高血脂、胰岛素抵抗和肝脂肪变性（P < 0.05）。然而，CCH-A 共同处理通过增加每日粪便脂质/胆汁酸的排泄、上调脂肪酸β氧化和下调肝脏脂肪酸生物合成，降低血清/肝脏甘油三酯水平和肝脏脂质堆积，从而改善胰岛素抵抗（P < 0.05）。CCH-A 共同处理还改善了胰岛素抵抗、增强了肝脏抗氧化能力、减少了肝损伤和炎症反应（P < 0.05）。总之，我们的研究结果不仅证明了 CCH-A 对慢性高脂肪饮食习惯的肝脏保护作用，其效果类似于辛伐他汀，而且还减轻了液体蛋产业处理卵黄系带所带来的环境负担。
