Department of Food Technology, Federal University of Technology - Paraná, Francisco Beltrão, PR, Brazil.
School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, RS, Brazil.
Food Res Int. 2021 Feb;140:110012. doi: 10.1016/j.foodres.2020.110012. Epub 2020 Dec 15.
Large amounts of by-products are generated during fish processing. The study aimed to assess whether tilapia scales are a potential source for obtaining glycosaminoglycans, as well as to determine their anticoagulant and cytotoxic/antiproliferative activities, against different tumor lines. The glycosaminoglycans were extracted, purified, and fractionated. The fractions that indicated the presence of uronic acid and sulfated GAGs were characterized by electrophoresis, NMR, and degree of sulfation (DS). The extraction process using the papain enzyme had a yield of 0.86%. Fraction V (FV) revealed the presence of chondroitin sulfate chains CS-A and CS-C, with DS of 0.146. FV demonstrated anticoagulant potential, as it was able to increase aPTT time. FV showed a cytotoxic effect for HTC metabolizing cells at 24, 48, and 72 h. However, it did not show activity for neuroblastoma cells in any of the evaluated times. The results indicate that the tilapia scales are a possible source for obtaining chondroitin sulfate, with potential use as anticoagulant and cytotoxic/antitumor.
在鱼类加工过程中会产生大量副产物。本研究旨在评估罗非鱼鳞片是否是获得糖胺聚糖的潜在来源，并确定其对不同肿瘤细胞系的抗凝和细胞毒性/抗增殖活性。糖胺聚糖被提取、纯化和分级。表明存在糖醛酸和硫酸化 GAG 的级分通过电泳、NMR 和硫酸化程度 (DS) 进行表征。使用木瓜蛋白酶的提取过程得率为 0.86%。第五级分 (FV) 显示存在硫酸软骨素链 CS-A 和 CS-C，DS 为 0.146。FV 显示出抗凝潜力，因为它能够增加 aPTT 时间。FV 在 24、48 和 72 小时对 HTC 代谢细胞显示出细胞毒性作用。然而，在评估的任何时间点，它对神经母细胞瘤细胞均没有活性。结果表明，罗非鱼鳞片是获得硫酸软骨素的可能来源，具有作为抗凝剂和细胞毒性/抗肿瘤的潜在用途。
