Programa de Pós-Graduação em Bioquímica e Biologia Molecular, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte, Parnamirim, RN, Brasil.
Braz J Med Biol Res. 2021 Jul 16;54(10):e10730. doi: 10.1590/1414-431X2020e10730. eCollection 2021.
Chondroitin sulfate (CS) is a type of glycosaminoglycan described as an antioxidant molecule that has been found in animal species such as fish. Tilapia (Oreochromis niloticus) represents an eco-friendly source of this compound, since its economical processing generates usable waste, reducing the negative environmental impact. This waste was used for CS extraction, purification, characterization by enzymatic degradation, and evaluation of its antioxidant effect. CS obtained from tilapia presented sulfation mainly at carbon 4 of galactosamine, and it was not cytotoxic at concentrations up to 200 µg/mL. Furthermore, 100 µg/mL of CS from tilapia reduced the levels of reactive oxygen species to 47% of the total intracellular reactive oxygen species level. The ability of CS to chelate metal ions in vitro also suggested an ability to react with other pathways that generate oxidative radicals, such as the Haber-Weiss reaction, acting intracellularly in more than one way. Although the role of CS from tilapia remains unclear, the pharmacological effects described herein indicate that CS is a potential molecule for further study of the relationship between the structures and functions of chondroitin sulfates as antioxidants.
硫酸软骨素（CS）是一种糖胺聚糖，被描述为抗氧化分子，已在鱼类等动物物种中发现。罗非鱼（Oreochromis niloticus）是这种化合物的一种环保来源，因为其经济加工产生可用的废物，减少了负面的环境影响。这种废物被用于 CS 的提取、纯化、酶解特性分析以及抗氧化作用的评估。从罗非鱼中获得的 CS 主要在半乳糖胺的碳 4 位硫酸化，其在高达 200μg/mL 的浓度下没有细胞毒性。此外，100μg/mL 的罗非鱼 CS 将细胞内总活性氧水平降低了 47%。CS 在体外螯合金属离子的能力也表明它能够与其他生成氧化自由基的途径（如 Haber-Weiss 反应）发生反应，在细胞内以不止一种方式发挥作用。尽管罗非鱼 CS 的作用尚不清楚，但本文所述的药理作用表明，CS 是进一步研究作为抗氧化剂的硫酸软骨素结构和功能之间关系的潜在分子。
