School of Life Sciences, Huaiyin Normal University, Huaian 223300, China.
School of Life Sciences, Huaiyin Normal University, Huaian 223300, China.
Food Res Int. 2021 Feb;140:110067. doi: 10.1016/j.foodres.2020.110067. Epub 2020 Dec 24.
Arabinoxylan (AX) extracted from wheat bran has attracted much attention due to its immunomodulatory activity. However, the molecular mechanisms underlying this activity remain unclear. In this study, we conducted a comprehensive transcriptional study to investigate genetic changes related to AX and identified 2325 differentially expressed genes (DEGs). Gene Ontology classification revealed that the DEGs were mainly enriched in a series of immune-related processes. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that immune-related pathways were significantly enriched in top 20 pathways, including the nuclear factor-kappa B (NF-κB) signaling pathway and the TNF signaling pathway. Validation using quantitative polymerase chain reaction analysis revealed dynamic changes in the mRNA levels of immune-related Cd40, Csf1, Csf2, Fas, IL-1β, IL-6, IL-5, Irf1, and Tnfaip3, which were significantly up-regulated in the AX-treated group. Moreover, AX treatment led to the up-regulation of the nuclear translocation of NF-κB and its upstream target proteins such as PDK1, Akt, IκB-α, and GSK-3β. The dataset compiled from this study provides valuable information for further research on the complex molecular mechanisms associated with AX and the identification of target genes.
从麦麸中提取的阿拉伯木聚糖（AX）因其免疫调节活性而备受关注。然而，其活性的分子机制尚不清楚。在这项研究中，我们进行了一项全面的转录组学研究，以研究与 AX 相关的遗传变化，并鉴定出 2325 个差异表达基因（DEGs）。基因本体论分类表明，DEGs 主要富集在一系列免疫相关过程中。京都基因与基因组百科全书通路分析显示，免疫相关通路在前 20 条通路中显著富集，包括核因子-κB（NF-κB）信号通路和 TNF 信号通路。使用定量聚合酶链反应分析进行验证，结果显示免疫相关基因 Cd40、Csf1、Csf2、Fas、IL-1β、IL-6、IL-5、Irf1 和 Tnfaip3 的 mRNA 水平发生了动态变化，在 AX 处理组中显著上调。此外，AX 处理导致 NF-κB 及其上游靶蛋白如 PDK1、Akt、IκB-α 和 GSK-3β 的核转位增加。本研究中编制的数据集为进一步研究 AX 相关的复杂分子机制和鉴定靶基因提供了有价值的信息。
