Department of Environmental Biotechnology, School of Environmental Sciences, Bharathidasan University, Tiruchirappalli, India.
Department of Biotechnology, Anna University BIT-Campus, Tiruchirappalli, India.
Food Res Int. 2021 Feb;140:110081. doi: 10.1016/j.foodres.2020.110081. Epub 2020 Dec 29.
Chronic hyperglycemia and oxidative stress promote non-enzymatic glycation that leads to the production of advanced glycation end products (AGEs). AGEs casue significant damage to physiological proteins which result in several complications. The scenario also corresponds to the chronic consumption of a diet rich in AGEs. Despite understanding these mechanisms at the molecular level, the discovery of new drugs for these complications is under progress. Natural compounds might have great therapeutic potential for treating glycative consequences. In view of this, the study aimed to evaluate fruit extracts of Hylocereus polyrhizus towards determining its phenolics and flavonoid contents, as well as assessing it's in vitro antiglycative potential through the use of multistage glycation markers (early, intermediate and end stage products of β-aggregation) in sugar-protein model. In vitro hypoglycemic activity of H. polyrhizus extracts was evaluated through α-amylase and α glucosidase inhibitory activities. In vitro antioxidant potential of the fruit extracts was also examined against different free radical types including DPPH and ABTS. Among the different in vitro assays performed, methanolic and acetone extracts of the fruit, with higher phenolics and flavonoid content, have exerted significant antiglycation and antioxidant activities than other extracts namely aqueous, ethanol, hydro-ethanol, hydro-methanol, and petroleum ether. Ultra-Performance Liquid Chromatography coupled with Electrospray Ionization Mass Spectrometry (UPLC-ESI-MS/MS) analysis was employed to identify active polyphenolics that may be responsible for the antiglycative potential of H. polyrhizus. The analysis revealed some high-profile compounds that have well documented for their therapeutic benefits. Additionally, In silico analysis also showed the possible connection between identified compounds and mechanisms of action. 4- Prenylresveratrol, Vicenin, and Luteolin had observed as effectively interact with target protein in molecular docking analysis. This suggests H. polyrhizus as a good source of anti-glycation and antioxidants that may have potential applications for the treatment and prevention of glycation associated diabetic and aging complications.
慢性高血糖和氧化应激促进非酶糖基化，导致晚期糖基化终产物 (AGEs) 的产生。AGEs 会对生理蛋白造成严重损害，从而导致多种并发症。这种情况也与长期摄入富含 AGEs 的饮食相对应。尽管在分子水平上已经了解了这些机制，但这些并发症的新药发现仍在进行中。天然化合物可能具有治疗糖基化后果的巨大治疗潜力。有鉴于此，本研究旨在评估火龙果果实提取物的酚类和类黄酮含量，并通过使用糖蛋白模型中的多阶段糖化标志物（β-聚集的早期、中期和晚期产物）评估其体外抗糖化潜力。通过α-淀粉酶和α-葡萄糖苷酶抑制活性评估火龙果提取物的体外降血糖活性。还检查了水果提取物的体外抗氧化潜力，以对抗不同的自由基类型，包括 DPPH 和 ABTS。在进行的不同体外测定中，具有较高酚类和类黄酮含量的果实的甲醇和丙酮提取物比其他提取物（水、乙醇、水-乙醇、水-甲醇和石油醚）表现出显著的抗糖化和抗氧化活性。采用超高效液相色谱-电喷雾电离质谱联用 (UPLC-ESI-MS/MS) 分析鉴定可能负责火龙果抗糖化潜力的活性多酚。分析显示了一些具有良好治疗功效的高知名度化合物。此外，计算机模拟分析还显示了鉴定化合物与作用机制之间的可能联系。4- 烯丙基白藜芦醇、vicenin 和木犀草素被观察到与分子对接分析中的靶蛋白有效相互作用。这表明火龙果是一种很好的抗糖化和抗氧化剂来源，可能具有治疗和预防与糖化相关的糖尿病和衰老并发症的潜在应用。
