采用纳升液相色谱-轨道阱质谱法对拉脱维亚收获的谷物和豆类中的霉菌毒素进行分析。

Mycotoxins in cereals and pulses harvested in Latvia by nanoLC-Orbitrap MS.

作者信息

Reinholds Ingars, Jansons Martins, Fedorenko Deniss, Pugajeva Iveta, Zute Sanita, Bartkiene Elena, Bartkevics Vadims

机构信息

Institute of Food Safety, Animal Health and Environment "BIOR", Riga, Latvia.

Faculty of Chemistry, University of Latvia, Riga, Latvia.

出版信息

Food Addit Contam Part B Surveill. 2021 Jun;14(2):115-123. doi: 10.1080/19393210.2021.1892204. Epub 2021 Mar 2.

DOI:10.1080/19393210.2021.1892204

PMID:33648416

Abstract

Twenty-seven mycotoxins in unprocessed cereals ( = 110) and pulses ( = 23) harvested in Latvia were analysed by nanoflow liquid chromatography combined with Orbitrap high-resolution mass spectrometry. One or more mycotoxins were found in 99% of the cereals and 78% of the pulses. Deoxynivalenol, zearalenone and T-2 and HT-2 toxins were prevalent in 9 to 86% of the cereals, mostly below their maximum levels as set by the European regulations. Non-regulated type A and B trichothecenes were prevalent in 5 to 87% of the cereals, at concentrations of 0.27-83 µg kg and 1.7-4,781 µg kg, respectively. Quantification of emerging mycotoxins was also provided. Enniatins were detected in 94% of the cereals (3.5-2,073 µg kg) and 13% of the pulses (4.4-17 µg kg). toxins were prevalent in 94% of the cereals at concentrations of 0.72-307 µg kg and in 39% of the pulses at 0.69-10 µg kg.

摘要

采用纳流液相色谱结合轨道阱高分辨率质谱法，对拉脱维亚收获的未加工谷物（n = 110）和豆类（n = 23）中的27种霉菌毒素进行了分析。在99%的谷物和78%的豆类中发现了一种或多种霉菌毒素。脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮以及T-2毒素和HT-2毒素在9%至86%的谷物中普遍存在，大多低于欧洲法规设定的最高限量。未受监管的A类和B类单端孢霉烯族毒素在5%至87%的谷物中普遍存在，浓度分别为0.27 - 83 μg/kg和1.7 - 4781 μg/kg。还提供了新出现霉菌毒素的定量分析结果。在94%的谷物（3.5 - 2073 μg/kg）和13%的豆类（4.4 - 17 μg/kg）中检测到恩镰孢菌素。毒素在94%的谷物中普遍存在，浓度为0.72 - 307 μg/kg，在39%的豆类中浓度为0.69 - 10 μg/kg。