Pleadin Jelka, Frece Jadranka, Lešić Tina, Zadravec Manuela, Vahčić Nada, Malenica Staver Mladenka, Markov Ksenija
a Laboratory for Analytical Chemistry , Croatian Veterinary Institute Zagreb , Zagreb , Croatia.
b Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.
Food Addit Contam Part B Surveill. 2017 Dec;10(4):268-274. doi: 10.1080/19393210.2017.1345991. Epub 2017 Jul 1.
The aim of this study was to investigate the occurrence of deoxynivalenol (DON) and zearalenone (ZEN) in unprocessed cereals and soybean sampled in 2014 and 2015 from different fields located in Croatian regions. A total of 306 samples were analysed for DON and 415 samples for ZEN concentrations using quantitative ELISA methods. In 2014, DON and ZEN were determined in all samples in the mean concentrations of 1,461 ± 2,265 µg/kg and 656 ± 853 µg/kg, respectively, while in 2015 these means were 2,687 ± 2,731 µg/kg and 1,140 ± 1,630 µg/kg, respectively. The cultivation year significantly (p < 0.05) influenced mycotoxin concentrations, whereas the influence of cultivation region was seen with ZEN for all cereals except soybean, and not seen with DON at all. A higher contamination determined during 2015 could be explained by high to extreme humidity evidenced in the period of cereals' growth and harvesting.
本研究的目的是调查2014年和2015年从克罗地亚不同地区采集的未加工谷物和大豆中脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）的污染情况。使用定量酶联免疫吸附测定法对总共306份样品进行了DON分析，对415份样品进行了ZEN浓度分析。2014年，所有样品中DON和ZEN的平均浓度分别为1461±2265μg/kg和656±853μg/kg，而2015年这些平均值分别为2687±2731μg/kg和1140±1630μg/kg。种植年份对霉菌毒素浓度有显著影响（p<0.05），除大豆外，种植区域对所有谷物的ZEN有影响，而对DON则完全没有影响。2015年确定的较高污染可能是由于谷物生长和收获期间出现的高湿度至极端湿度所致。
