Srogl M, Hochmannová J, Nesvera J, Stokrová J, Klégr M
Folia Microbiol (Praha). 1977;22(5):353-9. doi: 10.1007/BF02877670.
An auxotrophic strain of Escherichia coli with the recB recC sbcB genotype was transformed by chromosomal DNA of the prototrophic strain and by plasmid DNA carrying genes for antibiotic resistance (R1 drd19). The donor plasmid DNA obtained by cell lysis in the presence of Triton X-100 and subsequent centrifugation in a caesium chloride-ethidium bromide gradient was shown to have a circular molecule and to retain its completeness after penetration into the recipient. Experiments with mixtures or plasmid and chromosomal DNA indicate a competition between these two DNA types during the transformation reaction in the given system.
具有recB recC sbcB基因型的大肠杆菌营养缺陷型菌株，被原养型菌株的染色体DNA以及携带抗生素抗性基因（R1 drd19）的质粒DNA转化。通过在Triton X - 100存在下细胞裂解，随后在氯化铯 - 溴化乙锭梯度中离心获得的供体质粒DNA，显示为环状分子，并且在进入受体后保持其完整性。质粒和染色体DNA混合物的实验表明，在给定系统的转化反应过程中，这两种DNA类型之间存在竞争。
