Opt Lett. 2021 Mar 1;46(5):997-1000. doi: 10.1364/OL.417273.
Simultaneous imaging of complementary absorption and fluorescence contrasts with high spatial resolution is useful for biomedical studies. However, conventional dual-modal photoacoustic (PA) and fluorescence imaging systems require the use of acoustic coupling media due to the contact operation of PA imaging, which causes issues and complicates the procedure in certain applications such as cell imaging and ophthalmic imaging. We present a novel dual-modal imaging system which combines non-contact PA microscopy (PAM) based on PA remote sensing and fluorescence microscopy (FLM) into one platform. The system enables high lateral resolution of 2 and 2.7 µm for PAM and FLM modes, respectively. In vivo imaging of a zebrafish larva injected with a rhodamine B solution is demonstrated, with PAM visualizing the pigment and FLM revealing the injected rhodamine B.
同时成像互补的吸收和荧光对比具有高空间分辨率是有益的生物医学研究。然而,传统的双模态光声(PA)和荧光成像系统需要使用声耦合介质由于接触操作的 PA 成像,这会导致问题和复杂化的程序在某些应用程序,如细胞成像和眼科成像。我们提出了一种新的双模态成像系统,结合非接触式 PA 显微镜(PAM)基于 PA 远程传感和荧光显微镜(FLM)成一个平台。该系统能够实现高横向分辨率分别为 2 和 2.7 µm 的 PAM 和 FLM 模式。体内成像的斑马鱼幼虫注射罗丹明 B 溶液,用 PAM 可视化色素和 FLM 显示注射的罗丹明 B。
