Dashtaki Afsaneh, Mahjoub Soleiman, Zabihi Ebrahim, Pourbagher Roghayeh
Student Research Committee, Babol University of Medical Sciences, Babol, I. R. Iran.
Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, I. R. Iran.
Rep Biochem Mol Biol. 2020 Oct;9(3):338-347. doi: 10.29252/rbmb.9.3.338.
Some recent studies have reported anti-tumor activity for Thymol, but the findings are inconsistent. This study aimed to investigate and compare Thymol's effects on MCF-7 cancer cells and fibroblasts while treated with tert-Butyl hydroperoxide (t-BHP).
In the pre-treatment, MCF-7 and fibroblast cells were treated with various Thymol concentrations and incubated for 24 h. Then, t-BHP was added to a final concentration of 50 μM, and the cells were incubated for one h. In the post-treatment, cells were incubated first with 50 μM t-BHP for one h and then treated with Thymol. Cell viability was tested by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Thymol's antioxidant capacity was measured by DPPH and FRAP assays, and lipid peroxidation levels were determined by the TBARS method.
The thymol effects were dose-dependent, and despite their antioxidant properties, at concentrations of 100 µg/ml or more, increased t-BHP toxicity and reduced cancer cell viability. MTT assay result showed that pre-treatment and post-treatment with Thymol for 24 hours effectively reduced MCF-7 and fibroblast cell viability compared with the untreated control group. Both pre- and post-treatment of Thymol, normal fibroblast cell viability was significantly greater than that of the MCF-7 cells.
Our finding showed that Thymol appears to be toxic to MCF-7 cells at lower concentrations than fibroblasts after 24 hours of incubation. Pre-treatment with Thymol neutralized the oxidative effect of t-BHP in fibroblasts but was toxic for MCF-7 cells.
最近的一些研究报道了百里香酚的抗肿瘤活性，但结果并不一致。本研究旨在调查和比较百里香酚在叔丁基过氧化氢（t-BHP）处理下对MCF-7癌细胞和成纤维细胞的影响。
在预处理中，将MCF-7细胞和成纤维细胞用不同浓度的百里香酚处理，并孵育24小时。然后，加入t-BHP使其终浓度达到50μM，并将细胞再孵育1小时。在处理后，先将细胞用50μM t-BHP孵育1小时，然后用百里香酚处理。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法检测细胞活力。通过DPPH和FRAP法测定百里香酚的抗氧化能力，并通过TBARS法测定脂质过氧化水平。
百里香酚的作用呈剂量依赖性，尽管具有抗氧化特性，但在浓度为100μg/ml或更高时，会增加t-BHP的毒性并降低癌细胞活力。MTT分析结果表明，与未处理的对照组相比，百里香酚预处理和后处理24小时均有效降低了MCF-7细胞和成纤维细胞的活力。百里香酚预处理和后处理后，正常成纤维细胞的活力均显著高于MCF-7细胞。
我们的研究结果表明，孵育24小时后，百里香酚对MCF-7细胞的毒性似乎低于对成纤维细胞的毒性。百里香酚预处理可中和t-BHP对成纤维细胞的氧化作用，但对MCF-7细胞有毒性。
