Rio Clinic, Istanbul, Turkey.
ENT Department, Taksim Training and Education Hospital, Istanbul, Turkey.
Aesthetic Plast Surg. 2021 Aug;45(4):1843-1852. doi: 10.1007/s00266-021-02180-z. Epub 2021 Mar 1.
In this study, we investigated the effect of apocynin on fat graft survival in a rat model.
Twenty-one Wistar albino male rats were included in the study. The grafts taken from the inguinal area were applied to the nape of each rat. The rats were randomly divided into three groups. Apocynin+DMSO, DMSO and saline were administered intraperitoneally once daily for 14 days. After 90 days, the animals were sacrificed and the grafts were excised. The weights and volumes were measured and sent for histopathological evaluation. The weight, volume, survival rates of the grafts, the number of viable cells with the MTT test and pathological parameters, inflammation, vascularization, fibrosis, lipogranuloma and cyst formation, were evaluated.
Statistically significant differences were found between the groups in survival rates (p<0.05). In paired comparisons between the groups, the parameters of viable cells and survival rates were statistically significantly higher in apocynin group compared to the other groups (p<0.05). The survival rates relating to the weight and viable cell count in the DMSO group were statistically significantly higher than in the control group (p<0.05). However, no statistically significant difference was found between the DMSO group and the control group in the survival rate in terms of volume (p<0.05). There was no significant difference between the groups in the comparison of pathological parameters (p>0.05).
Apocynin increases fat graft survival in the animal fat grafting model. Therefore, apocynin can be used as an effective medical agent to prevent the volume loss of fat grafts.
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本研究旨在探讨白杨素对大鼠脂肪移植存活的影响。
本研究纳入 21 只 Wistar 白化雄性大鼠。将取自腹股沟区域的移植物应用于每只大鼠的项背部。大鼠随机分为三组。白杨素+DMSO、DMSO 和生理盐水分别每天腹腔内给药 14 天。90 天后，处死动物并切除移植物。测量移植物的重量和体积，并进行组织病理学评估。评估移植物的重量、体积、存活率、MTT 试验中活细胞数量和病理参数、炎症、血管生成、纤维化、脂肪肉芽肿和囊肿形成。
各组间存活率差异有统计学意义（p<0.05）。组间配对比较显示，与其他组相比，白杨素组活细胞数和存活率参数均显著更高（p<0.05）。DMSO 组的存活率与重量和活细胞计数相关，明显高于对照组（p<0.05）。然而，在体积方面，DMSO 组与对照组之间的存活率无统计学差异（p<0.05）。各组间病理参数比较无统计学差异（p>0.05）。
白杨素可提高动物脂肪移植模型中脂肪移植物的存活率。因此，白杨素可用作预防脂肪移植物体积损失的有效药物。
本杂志要求作者为每篇文章分配一个证据级别。有关这些循证医学评级的完整描述，请参阅目录或在线作者指南 www.springer.com/00266 。
