Department of Medicine and Surgery, University of Parma, Parma, Italy.
Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.
Methods Mol Biol. 2021;2278:61-70. doi: 10.1007/978-1-0716-1274-3_6.
Bifidobacteria are commensal microorganisms able to colonize several ecological niches. Since their discovery, culture-dependent methods combined with the most modern next-generation sequencing techniques have contributed to shed light on the ecological, functional and genomic features of bifidobacteria, purporting them as microorganisms with probiotic traits. Thanks to their acclaimed health-promoting effects, several members of the Bifidobacterium genus have been included in a variety of functional foods and drugs. In this context, the functional relevance of bifidobacteria in the gut explains ongoing efforts to isolate novel and potentially beneficial strains. For this purpose, development of effective and selective isolation protocols in concert with knowledge on the physiological characteristics of bifidobacterial are fundamental requirements for their recovery and discovery from their natural environments, in particular from fecal samples.
双歧杆菌是能够定殖于多个生态位的共生微生物。自从它们被发现以来，基于培养的方法结合最现代的下一代测序技术，有助于揭示双歧杆菌的生态、功能和基因组特征，使其成为具有益生菌特性的微生物。由于它们备受赞誉的促进健康的作用，双歧杆菌属的几个成员已被纳入各种功能性食品和药物中。在这种情况下，双歧杆菌在肠道中的功能相关性解释了人们正在努力分离新型的潜在有益菌株。为此，开发有效的和选择性的分离方案，结合双歧杆菌生理特性的知识，对于从其自然环境中（特别是从粪便样本中）恢复和发现它们是基本要求。
