College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong, People's Republic of China.
Food Chem. 2020 Nov 1;329:127179. doi: 10.1016/j.foodchem.2020.127179. Epub 2020 Jun 1.
The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1-10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation. The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption. GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect. Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate. During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation. Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1. Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.
采用体外发酵法评估了水解瓜尔胶（GMOS，<1 kDa；GMPS，1-10 kDa）、甘露低聚糖（MOS，<1 kDa）和半乳糖低聚糖（GOS，<1 kDa）的益生元活性。测试的碳水化合物对细菌生长、短链脂肪酸（SCFA）产生和底物消耗具有选择性的益生元作用。GOS 和 GMOS 分别显著促进双歧杆菌和丁酸梭菌的生长，而 MOS 表现出最强的产丁酸作用。此外，水解瓜尔胶组的 SCFA 产生与水解产物的变化分子量（Mw）密切相关。在使用人粪便接种物的体外发酵中，GMOS 在 48 h 发酵后产生的乳酸盐、丙酸盐和丁酸盐产量最高。MOS 和 C. butyricum 的联合应用增加了梭菌科_1 的丰度。总的来说，我们的结果表明，半乳糖基和甘露糖基碳水化合物具有个性化的益生元作用，这与其化学结构有关，包括糖苷组成和 Mw。
