Laboratory of Tropical Veterinary Medicine and Vector Biology, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228, Hainan, China.
One Health Institute, Hainan Univeristy, Haikou, 570228, Hainan, China.
Parasitol Res. 2021 May;120(5):1799-1809. doi: 10.1007/s00436-021-07077-z. Epub 2021 Mar 2.
Present-day diagnostic tools and technologies for canine diseases and other vector-borne parasitic diseases hardly meet the requirements of an efficient and rapid diagnostic tool, which can be suitable for use at the point-of-care in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been always a method of choice in the development and validation of quick, precise, and sensitive diagnostic assays for pathogen detection and to reorganize point-of-care (POC) molecular diagnostics. In this study, we have demonstrated an efficient detection system for parasitic vector-borne pathogens like Ehrlichia canis and Hepatozoon canis by linking the LAMP assay to a smartphone via a simple, inexpensive, and a portable "LAMP box," All the components of the LAMP box were connected to each other wirelessly. This LAMP box was made up of an isothermal heating pad mounted below an aluminum base which served as a platform for the reaction tubes and LAMP assay. The entire setup could be connected to a smartphone via an inbuilt Wi-Fi that allowed the user to establish the connection to control the LAMP box. A 5 V USB power source was used as a power supply. The sensitivity of the LAMP assay was estimated to be up to 10 dilution limit using the amplified, purified, and quantified specific DNA templates. It can also serve as an efficient diagnostic platform for many other veterinary infectious or parasitic diseases of zoonotic origin majorly towards field-based diagnostics.
目前用于犬病和其他媒介传播寄生虫病的诊断工具和技术很难满足高效快速诊断工具的要求，这种诊断工具适用于资源有限环境下的现场即时诊断。环介导等温扩增（LAMP）技术一直是用于开发和验证快速、准确、灵敏的病原体检测诊断检测方法的首选方法，以重新组织现场即时（POC）分子诊断。在这项研究中，我们通过一种简单、廉价且便携式的“LAMP 盒”将 LAMP 检测与智能手机连接起来，证明了一种针对寄生虫媒介传播病原体（如犬埃立克体和犬巴贝斯虫）的高效检测系统。LAMP 盒的所有组件都通过无线方式相互连接。该 LAMP 盒由安装在铝制底座下方的等温加热垫组成，铝制底座用作反应管和 LAMP 检测的平台。整个装置可以通过内置 Wi-Fi 连接到智能手机，用户可以通过该 Wi-Fi 建立连接来控制 LAMP 盒。5V USB 电源用作电源。通过扩增、纯化和定量特定的 DNA 模板，LAMP 检测的灵敏度估计可达 10 倍稀释极限。它还可以作为一个高效的诊断平台，用于许多其他源自动物的兽医传染病或寄生虫病的现场诊断。
