Sadeghzadeh Oskouei Behnaz, Zargari Siavash, Shahabi Parviz, Ghaffari Novin Marefat, Pashaiasl Maryam
Department of Midwifery, School of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Electrical and Computer Engineering, University of Tabriz, Tabriz, Iran.
Cell J. 2021 Apr;23(1):32-39. doi: 10.22074/cellj.2021.7056. Epub 2021 Mar 1.
In customary assisted reproductive technology (ART), oocyte culture occurs in static micro drops of Petri dishes with vast media volume; while, the condition is dynamic. In this study, we aimed to improve the maturation efficiency of mammalian oocytes by designing an optimal microchamber array to obtain the integration of oocyte trapping and maturation within a microfluidic device and evaluate the role of microfluidic culture condition in lipid peroxidation level of the culture medium, matured oocytes apoptosis, and its comparison with the conventional static system.
In this experimental research, immature oocytes were collected from ovaries of the Naval Medical Research Institute (NMRI) mice. Oocytes were randomly laid in static and dynamic (passive and active) maturation culture medium for 24 hours. The lipid peroxidation level in oocyte culture media was assessed by measuring the concentration of malondialdehyde (MDA), and the rate of apoptosis in matured oocytes was assessed by the TUNEL assay after a-24 hour maturation period.
The MDA concentration in both dynamic oocyte maturation media were significantly lower than the static medium (0.003 and 0.002 vs. 0.13 μmol/L, P<0.01). Moreover, the rate of apoptosis in matured oocytes after a-24 hour maturation period was significantly lower in passive dynamic and active dynamic groups compared with the static group (16%, 15% vs. 35%, P<0.01).
The dynamic culture for oocyte maturation (IVM) improves the viability of IVM oocytes in comparison with the static culture condition.
在传统辅助生殖技术（ART）中，卵母细胞培养是在培养皿的静态微滴中进行，培养基体积庞大；而实际情况是动态的。在本研究中，我们旨在通过设计一种最佳的微腔阵列来提高哺乳动物卵母细胞的成熟效率，以实现卵母细胞捕获与在微流控装置内成熟的整合，并评估微流控培养条件对培养基脂质过氧化水平、成熟卵母细胞凋亡的作用，以及与传统静态系统的比较。
在本实验研究中，从未成熟的海军医学研究所（NMRI）小鼠卵巢中收集卵母细胞。将卵母细胞随机置于静态和动态（被动和主动）成熟培养基中培养24小时。通过测量丙二醛（MDA）浓度评估卵母细胞培养基中的脂质过氧化水平，在24小时成熟培养期后，通过TUNEL法评估成熟卵母细胞的凋亡率。
两种动态卵母细胞成熟培养基中的MDA浓度均显著低于静态培养基（0.003和0.002 vs. 0.13 μmol/L，P<0.01）。此外，与静态组相比，在24小时成熟培养期后，被动动态组和主动动态组成熟卵母细胞的凋亡率显著降低（16%，15% vs. 35%，P<0.01）。
与静态培养条件相比，卵母细胞成熟的动态培养（IVM）提高了IVM卵母细胞的活力。
