Curylofo Patrícia Curylofo, Raile Priscilla Neves, Oliveira Viviane de Cássia, Macedo Ana Paula, Guedes Débora Fernandes Costa, Paranhos Helena de Freitas Oliveira, Pagnano Valéria Oliveira
Int J Prosthodont. 2023 Nov 1;36(5):650. doi: 10.11607/ijp.7264.
To evaluate the application of chitosan as a cleanser in the control of biofilm formation on cobalt-chromium (Co-Cr) alloy and acrylic resin surfaces.
In total, 172 Co-Cr discs and 172 acrylic resin discs (14 mm x 3 mm) were contaminated with Streptococcus mutans, Staphylococcus aureus, Candida albicans, or Candida glabrata and incubated for 48 hours. Then, specimens were randomly divided into groups and immersed in the following solutions for 15 minutes: solution without chitosan (WC/control); chitosan solution (CH: 5 mg/mL); chitosan nanoparticle solu.on (CN: 3.8 mg/mL); and effervescent tablet (ET). Biofilm recovery rates (n = 9) were evaluated by counting the colony-forming units (CFU/mL). Biofilm morphology was evaluated using scanning electron microscopy (SEM). Data were compared using the Kruskal-Wallis or ANOVA tests followed by the Tukey post hoc test.
For acrylic resin, ET showed the lowest number of CFU for S aureus and S mutans (P < .001). CH exhibited intermediate values for S mutans, S aureus, and C albicans; CN exhibited intermediate values for S mutans and S aureus. For C glabrata, there was no sta.s.cal difference between the solu.ons (P = .264). For Co-Cr, ET showed the highest level of antimicrobial action against all microorganisms (P < .001), and CH showed an intermediate level of action against S mutans and S aureus. Against C albicans and C glabrata, there was no significant difference among CH, CN, and WC.
Although ET had a broader spectrum of antimicrobial action, CH showed promise as a denture cleanser. Int J Prosthodont 2023;36:e61-e73.
评估壳聚糖作为清洁剂在控制钴铬（Co-Cr）合金和丙烯酸树脂表面生物膜形成方面的应用。
总共172个Co-Cr圆盘和172个丙烯酸树脂圆盘（14毫米×3毫米）被变形链球菌、金黄色葡萄球菌、白色念珠菌或光滑念珠菌污染，并孵育48小时。然后，将标本随机分组并浸入以下溶液中15分钟：不含壳聚糖的溶液（WC/对照）；壳聚糖溶液（CH：5毫克/毫升）；壳聚糖纳米颗粒溶液（CN：3.8毫克/毫升）；以及泡腾片（ET）。通过计数菌落形成单位（CFU/毫升）评估生物膜回收率（n = 9）。使用扫描电子显微镜（SEM）评估生物膜形态。数据采用Kruskal-Wallis或方差分析检验，随后进行Tukey事后检验进行比较。
对于丙烯酸树脂，ET对金黄色葡萄球菌和变形链球菌的CFU数量最低（P <.001）。CH对变形链球菌、金黄色葡萄球菌和白色念珠菌表现出中间值；CN对变形链球菌和金黄色葡萄球菌表现出中间值。对于光滑念珠菌，各溶液之间无统计学差异（P =.264）。对于Co-Cr，ET对所有微生物的抗菌作用水平最高（P <.001），CH对变形链球菌和金黄色葡萄球菌表现出中等作用水平。对于白色念珠菌和光滑念珠菌，CH、CN和WC之间无显著差异。
尽管ET具有更广泛的抗菌作用谱，但CH有望作为假牙清洁剂。《国际口腔修复学杂志》2023年；36：e61-e73。
