Department of Dental Materials and Prosthodontics, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Human Exposome and Infectious Diseases Network-HEID, School of Nursing of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Gerodontology. 2023 Dec;40(4):472-483. doi: 10.1111/ger.12672. Epub 2023 Jan 11.
To evaluate the anti-biofilm action of chitosan, nanoparticulate chitosan, and denture cleanser Nitradine™ against biofilms comprising Candida albicans, Candida glabrata, Staphylococcus aureus, and Streptococcus mutans.
Biofilm removal from removable partial dentures (RPD) is important for success in prosthetic rehabilitation.
The anti-biofilm action of the experimental chitosan-based solutions and Nitradine™ was evaluated on acrylic resin and cobalt-chromium alloy through assessing cell viability, cell metabolism, residual aggregated biofilm, and extracellular polymeric substance and biofilm morphology.
Only chitosan reduced the viability of C. albicans on cobalt-chromium alloy surface, by 98% (a 1.7 log10 reduction in cfu). Chitosan-based solutions neither promoted substantial alteration of the metabolic activity of the four-species biofilm nor reduced the amount of the aggregated biofilm. After immersion in chitosan and nanoparticulate chitosan, viable microorganisms and extracellular polymeric substances distributed over the entire specimens' surfaces were observed. Nitradine™ reduced the viability and metabolic activity of biofilm grown on both surfaces, but it did not remove all aggregated biofilm and extracellular polymeric substances. After immersion in Nitradine™, approximately 35% of the specimens' surfaces remained covered by aggregated biofilm, mainly composed of dead cells.
Although chitosan and Nitradine™ promoted changes in the viability of microorganisms, neither solution completely removed the four-species biofilm from the Co-Cr and acrylic resin surfaces. Thus, isolated use of hygiene solutions is not indicated for biofilm control on RPDs; this requires complementary mechanical removal.
评估壳聚糖、纳米壳聚糖和义齿清洁剂 Nitradine™ 对包含白色念珠菌、近平滑念珠菌、金黄色葡萄球菌和变形链球菌的生物膜的抗生物膜作用。
从可摘局部义齿 (RPD) 上去除生物膜对于修复体成功非常重要。
通过评估细胞活力、细胞代谢、残留聚集生物膜、细胞外聚合物和生物膜形态，评估了基于实验壳聚糖的溶液和 Nitradine™ 对丙烯酸树脂和钴铬合金的抗生物膜作用。
只有壳聚糖降低了白色念珠菌在钴铬合金表面的活力，降低了 98%（cfu 减少了 1.7 对数）。基于壳聚糖的溶液既没有促进四种生物膜的代谢活性发生实质性改变，也没有减少聚集生物膜的量。在壳聚糖和纳米壳聚糖浸泡后，观察到活微生物和细胞外聚合物分布在整个标本表面。Nitradine™ 降低了两种表面上生长的生物膜的活力和代谢活性，但它没有去除所有聚集的生物膜和细胞外聚合物。浸泡在 Nitradine™ 后，大约 35%的标本表面仍被聚集的生物膜覆盖，主要由死细胞组成。
尽管壳聚糖和 Nitradine™ 促进了微生物活力的变化，但两种溶液都不能从 Co-Cr 和丙烯酸树脂表面完全去除四种生物膜。因此，单独使用卫生溶液不适合控制 RPD 上的生物膜；这需要补充机械去除。
