Department of Prosthodontics, Paulista University, Goiânia, GO, Brazil.
J Appl Oral Sci. 2012 Nov-Dec;20(6):643-8. doi: 10.1590/s1678-77572012000600009.
The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation.
Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (10(7) CFU/mL) and incubated at 37ºC for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37ºC for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05.
The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079).
Acrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm.
本研究旨在评估一种丙烯酸树脂与抗菌聚合物聚（2-叔丁基氨基乙基）甲基丙烯酸酯（PTBAEMA）结合，以抑制金黄色葡萄球菌、变形链球菌和白色念珠菌生物膜形成的抗菌活性。
制作热聚合丙烯酸树脂圆盘，并根据 PTBAEMA 浓度进行分组：0（对照）、10 和 25%。将标本接种（10（7）CFU/mL）并在 37°C 孵育 48 小时。孵育后，用无菌生理盐水冲洗孔，然后将每个标本用超声处理 20 分钟。将得到的悬浮液等分试样在 37°C 下培养 48 小时。对菌落形成单位（CFU）进行计数，并以 log（CFU+1）/mL 表示，用α=0.05 进行统计学分析。
结果表明，25%PTBAEMA 完全抑制了金黄色葡萄球菌和变形链球菌的生物膜形成。含有 10%PTBAEMA 的组中，金黄色葡萄球菌（对照：7.9±0.8A；10%：3.8±3.3B）和变形链球菌（对照：7.5±0.7A；10%：5.1±2.7B）的 log（CFU+1）/mL 计数显著减少（Mann-Whitney，p<0.05）。对于白色念珠菌，各组之间差异无统计学意义（对照：6.6±0.2A；10%：6.6±0.4A；25%：6.4±0.1A）（Kruskal-Wallis，p>0.05，P=0.079）。
丙烯酸树脂与 10%和 25%的 PTBAEMA 结合具有抑制金黄色葡萄球菌和变形链球菌生物膜的显著抗菌活性，但对白色念珠菌生物膜无效。
