Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Korea.
Anal Methods. 2021 Mar 21;13(11):1332-1339. doi: 10.1039/d1ay00074h. Epub 2021 Mar 2.
In this study, we report on paper-based colorimetric detection of sulfide using a newly synthesized chemical acting as a chemosensor, based on the deprotonation mechanism. Paper strips were also fabricated and incorporated with the chemosensor for on-site monitoring. The presence of sulfide induced deprotonation of a hydroxyl group of the chemosensor, which eventually resulted in a distinct spectral change in the tube as well as a visible color change on a paper strip. The chemosensor showed a highly selective colorimetric response to sulfide by changing its color from colorless to yellow without any interference from a mixture containing other anions. Moreover, the chemosensor effectively differentiated sulfide from other thiols, including cysteine and glutathione. The chemosensor colorimetrically detected sulfide with a fast response time of 10 s under physiological conditions. Practically, the paper test strip enabled colorimetric visualization of as low as 30 μM sulfide and a good recovery in quantitative analysis in water samples. The introduced paper-based chemosensor is a promising colorimetric strategy with rapid, selective, and sensitive sensing abilities for sulfide monitoring in environmental water samples.
在这项研究中，我们报告了一种新合成的化学物质作为化学传感器，基于去质子化机制，用于基于纸张的比色检测硫化物。还制备了纸条，并将其与化学传感器结合，用于现场监测。硫化物的存在诱导了化学传感器上一个羟基的去质子化，最终导致管中光谱发生明显变化，纸条上也发生可见颜色变化。该化学传感器对硫化物具有高度选择性的比色响应，其颜色从无色变为黄色，而不受包含其他阴离子的混合物的干扰。此外，该化学传感器能够有效地将硫化物与其他硫醇（包括半胱氨酸和谷胱甘肽）区分开来。在生理条件下，该化学传感器的比色检测硫化物具有快速的响应时间（10 秒）。实际上，纸条测试条能够实现低至 30 μM 的硫化物的比色可视化，并且在水样中的定量分析中具有良好的回收率。引入的基于纸张的化学传感器是一种很有前途的比色策略，具有快速、选择性和敏感的传感能力，可用于环境水样中的硫化物监测。
