Hansen Victoria L, Kahl Stanislaw, Proszkowiec-Weglarz Monika, Jiménez Stephanie C, Vaessen Stefan F C, Schreier Lori L, Jenkins Mark C, Russell Beverly, Miska Katarzyna B
Animal Biosciences and Biotechnology Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705, USA.
Perstorp, 211 20 Malmo, Sweden.
Poult Sci. 2021 Apr;100(4):100984. doi: 10.1016/j.psj.2021.01.007. Epub 2021 Jan 18.
Butyrate is a feed additive that has been shown to have antibacterial properties and improve gut health in broilers. Here, we examined the performance and gene expression changes in the ileum of tributyrin-supplemented broilers infected with coccidia. Ninety-six, Ross 708 broilers were fed either a control corn-soybean-based diet (-BE) or a diet supplemented with 0.25% (w/w) tributyrin (+BE). Birds were further divided into groups that were inoculated with Eimeria maxima oocysts (EM) or sham-inoculated (C) on day 21 posthatch. At 7 d postinfection (7 d PI), the peak of pathology in E. maxima infection, tributyrin-supplemented birds had significantly improved feed conversion ratios (FCR, P < 0.05) and body weight gain (BWG, P < 0.05) compared with -BE-infected birds, despite both groups having similar feed intake (FI, P > 0.05). However, at 10 d post-infection (10 d PI) no significant effects of feed type or infection were observed. Gene expression in the ileum was examined for insights into possible effects of infection and tributyrin supplementation on genes encoding proteins related to immunity, digestion, and gut barrier integrity. Among immune-related genes examined, IL-1B and LEAP2 were only significantly affected at 7 d PI. Transcription of genes related to digestion (APN, MCT1, FABP2, and MUC2) were primarily influenced by infection at 7 d PI and tributyrin supplementation (FABP2 and MUC2) at 10 d PI. With exception of ZO1, tight junction genes were affected by either infection or feed type at 7 d PI. At 10 d PI, only CLDN1 was not affected by either infection or feed type. Overall tributyrin shows promise as a supplement to improve performance during coccidiosis in broiler chickens; however, its effect on gene expression and mode of action requires further research.
丁酸盐是一种饲料添加剂，已被证明具有抗菌特性，并能改善肉鸡的肠道健康。在此，我们研究了感染球虫的添加甘油三丁酸酯的肉鸡回肠的性能和基因表达变化。96只罗斯708肉鸡分别饲喂基于玉米-大豆的对照日粮（-BE）或添加0.25%（w/w）甘油三丁酸酯的日粮（+BE）。在孵化后第21天，将鸡进一步分为接种巨型艾美耳球虫卵囊（EM）的组或假接种（C）的组。在感染后7天（PI），即巨型艾美耳球虫感染病理学的高峰期，与感染-BE的鸡相比，添加甘油三丁酸酯的鸡的饲料转化率（FCR，P<0.05）和体重增加（BWG，P<0.05）显著改善，尽管两组的采食量相似（FI，P>0.05）。然而，在感染后10天（PI），未观察到饲料类型或感染的显著影响。检测回肠中的基因表达，以深入了解感染和添加甘油三丁酸酯对编码与免疫、消化和肠道屏障完整性相关蛋白质的基因的可能影响。在所检测的免疫相关基因中，IL-1B和LEAP2仅在感染后7天受到显著影响。与消化相关的基因（APN、MCT1、FABP2和MUC2）的转录主要在感染后7天受感染影响，在感染后10天受甘油三丁酸酯添加（FABP2和MUC2）影响。除ZO1外，紧密连接基因在感染后7天受感染或饲料类型影响。在感染后10天，只有CLDN1不受感染或饲料类型影响。总体而言，甘油三丁酸酯有望作为一种补充剂，改善肉鸡球虫病期间的性能；然而，其对基因表达的影响和作用模式需要进一步研究。
