Department of Animal Resource and Science, Dankook University, No. 29 Anseodong, Cheonan, Choongnam 330-714, Korea.
DSM Nutrition Korea Ltd. 91 Bangbaecheon-ro, Seocho-ku, Seoul 06675, Republic of Korea.
Poult Sci. 2019 Jul 1;98(7):2919-2926. doi: 10.3382/ps/pez040.
A total of 800 1-day-old Ross 308 male broiler chicks were used in a 5-wk feeding trial to evaluate the effects of commercial feed additive that is composed of blends of essential oil (EO; CRINA Poultry, DSM) and vitamin D3. The birds were randomly assigned to 5 treatments with 10 replicate cages; each stocked with 16 birds and was fed corn-soybean-meal-based basal diet. Dietary treatments included: CON, unvaccinated birds fed basal diet/control; CCO, CON + coccidian oocysts vaccinated birds; EOD1, CCO + Essential oil blends and vitamin D/Prototype 1 (50 μg 25-OH-D3/kg diet, 15 mg CRINA Poultry/kg diet); EOD2, CCO + Prototype 2 (69 μg 25-OH-D3/kg diet, 30 mg CRINA Poultry/kg diet); and EOD3, CCO + Prototype 3 (138 μg 25-OH-D3/kg diet, 50 mg CRINA Poultry/kg diet). On day 7, all birds, except CON, were inoculated with 300 to 500 purified sporulated viable oocysts from Eimeria tenella and Eimeria maxima. As a result of this study, the supplementation of EO blends with vitamin D3 showed trends in improvement (P = 0.075) body weight gain (BWG) compared with CON and CCO during the grower phase (day 7 to 21). An increase (P < 0.05) in BWG and reduction (P < 0.05) in feed conversion ratio (FCR) were observed in birds fed EOD2 diet during day 21 to 35 compared with vaccinated birds without feed additive. The cumulative performance during overall period (day 1 to 35) also showed that BWG was increased and FCR was reduced in EOD2 and EOD3 compared with CON, CCO, and EOD1. Furthermore, the apparent total tract digestibility of dry matter tended (P = 0.06) to increase in EOD2 and EOD3 compared with CON and other treatments. The EOD2 and EOD3 treatments showed increased (P < 0.05) excreta Lactobacillus counts than EOD1. The intestinal lesion score induced by E. tennela in gastro-intestinal tract was lower (P < 0.05) in EOD1, EOD2, and EOD3 treatments compared with CCO. In conclusion, blends of EO and vitamin D3 could be used as anti-coccidial feed additive in broilers with coccidian infection.
本试验旨在评估一种由植物精油（CRINA Poultry，DSM）和维生素 D3 混合物组成的商业饲料添加剂对罗斯 308 肉鸡的影响。选用 800 只 1 日龄雄性罗斯 308 肉鸡进行为期 5 周的饲养试验，将其随机分为 5 个处理组，每个处理组设 10 个重复，每个重复 16 只鸡，采用玉米-豆粕型基础日粮进行饲养。试验日粮处理如下：CON：未接种疫苗、饲喂基础日粮的对照组；CCO：CON+球虫卵囊接种组；EOD1：CCO+植物精油混合物和维生素 D/原型 1（25-羟维生素 D3 50μg/kg 日粮，CRINA Poultry 15mg/kg 日粮）；EOD2：CCO+原型 2（25-羟维生素 D3 69μg/kg 日粮，CRINA Poultry 30mg/kg 日粮）；EOD3：CCO+原型 3（25-羟维生素 D3 138μg/kg 日粮，CRINA Poultry 50mg/kg 日粮）。第 7 天，除 CON 组外，所有鸡均接种 300500 个纯化的、有活力的柔嫩艾美耳球虫和毒害艾美耳球虫卵囊。结果表明，与 CON 和 CCO 组相比，在生长阶段（第 721 天），添加维生素 D3 的精油混合物对体重增加（BWG）有改善趋势（P=0.075）。与未接种疫苗的鸡相比，EOD2 组在第 2135 天的 BWG 增加（P<0.05），饲料转化率（FCR）降低（P<0.05）。在整个试验期间（第 135 天），EOD2 和 EOD3 组的累积性能也表明，与 CON、CCO 和 EOD1 相比，BWG 增加，FCR 降低。此外，EOD2 和 EOD3 组的干物质表观全肠道消化率呈增加趋势（P=0.06）。EOD2 和 EOD3 组的粪便乳酸菌数量比 EOD1 组增加（P<0.05）。E. tennela 诱导的胃肠道肠黏膜损伤评分在 EOD1、EOD2 和 EOD3 组中低于 CCO 组（P<0.05）。综上所述，植物精油和维生素 D3 混合物可作为感染球虫病肉鸡的抗球虫饲料添加剂。
