Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Science, Changchun 130102, China; Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education of China, Northwest A & F University, Yangling, China.
Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Sci Total Environ. 2021 Jul 1;776:146029. doi: 10.1016/j.scitotenv.2021.146029. Epub 2021 Feb 22.
To explore the potential association between the diversity of endophytic microorganisms and modifications of grain quality in wheat exposed to multi-generational elevated CO concentration, the grain quality attributes and microbial diversity were tested after five generations successively grown in ambient CO concentration (F5_A, 400 μmol L) and elevated CO concentration (F5_E, 800 μmol L). Elevated CO concentration significantly increased the grain number and starch concentration, while decreased the grain protein concentration. Multi-generational exposure to elevated CO concentration also led to significant changes in grain amino acid concentration. In response to the elevated CO concentration, Pseudomonas, Rhodococcus, Ralstonia, and Klebsiella were the dominant bacterial genera, while Penicillium, Cutaneotrichosporon, Fusarium, Sarocladium, Acremonium and Aspergillus were the dominant fungal genera in wheat grain. A significantly positive correlation was found between Pseudomonas, Penicillium and ratio of starch to protein concentration, implying that the multi-generational CO elevation induced modifications in grain quality might be associated with the changes in grain microbial diversity. The results of this study suggest that the endophytic microbes may play an important role in modulating the grain nutritional quality in wheat under multi-generational e[CO] exposure, through regulating starch and N metabolism and production of secondary metabolites.
为了探究在多代大气 CO 浓度升高的环境下，内生微生物多样性与小麦籽粒品质变化之间的潜在关联，我们对经过五代数代培养在大气 CO 浓度（F5_A，400 μmol·L）和升高的 CO 浓度（F5_E，800 μmol·L）下的小麦籽粒品质属性和微生物多样性进行了测试。升高的 CO 浓度显著增加了籽粒数量和淀粉浓度，而降低了籽粒蛋白浓度。多代数代暴露于升高的 CO 浓度也导致了籽粒氨基酸浓度的显著变化。对升高的 CO 浓度的响应，假单胞菌、红球菌、罗尔斯通氏菌和克雷伯氏菌是优势细菌属，而青霉属、毛孢子菌属、镰刀菌属、Sarocladium 属、枝孢属和曲霉属是小麦籽粒中的优势真菌属。假单胞菌、青霉属与淀粉与蛋白浓度比值之间存在显著正相关，这表明多代数代 CO 升高引起的籽粒品质变化可能与籽粒微生物多样性的变化有关。本研究结果表明，内生微生物可能通过调节淀粉和 N 代谢以及次生代谢产物的产生，在小麦多代数代[CO]暴露下调节籽粒营养品质方面发挥重要作用。
