Sequence of a human kidney cDNA clone encoding thymosin beta 10.

We have isolated a cDNA clone encoding the 43 amino acid protein, thymosin beta 10, from an adult human kidney cDNA library. A comparison of human and rat thymosin beta 10 cDNA sequences revealed 100% identity for the deduced amino acid sequence and 95% nucleotide identity for the coding region. The thymosin beta 10 cDNA clone was used to probe RNA isolated from monkey kidney epithelial cells of the BSC-1 line. The probe recognized 850 bp and 2.4 kb transcripts in quiescent cells which indicates that the gene is constitutively expressed. The abundance of the 2.4 kb transcript was markedly increased one hour after serum stimulation and declined progressively to a basal value at 24 hours.