Koprivova Anna, Schmalenberger Achim, Kopriva Stanislav
Botanical Institute and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, Germany.
Department of Biological Sciences, University of Limerick, Limerick, Ireland.
Bio Protoc. 2020 Jan 20;10(2):e3490. doi: 10.21769/BioProtoc.3490.
Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew the plants in soil/sand mix under control conditions and measured the sulfatase activity in soil using a spectrophotometric determination of the product. This protocol can be used to test the contribution of individual genes to control of microbiome assembly through analysis of mutants as well as the influence of environment on plant-microbe interactions.
硫酸酯酶活性常被用作衡量土壤微生物活性的指标。因此，它是研究微生物对植物反应的合适工具。在此，我们介绍一种利用硫酸酯酶作为定量指标来确定各种基因型对土壤微生物群功能影响的方法。我们在可控条件下将植物种植于土壤/沙子混合物中，并通过分光光度法测定产物来测量土壤中的硫酸酯酶活性。该方案可用于通过分析突变体来测试单个基因对微生物群落组装控制的贡献，以及环境对植物-微生物相互作用的影响。
