麻醉状态下的胰岛素耐量试验以测量组织特异性胰岛素刺激的葡萄糖处置情况。
Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.
作者信息
Fazakerley Daniel J, Fritzen Andreas M, Nelson Marin E, Thorius Ida H, Cooke Kristen C, Humphrey Sean J, Cooney Gregory J, James David E
机构信息
Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia.
Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen 2200, Denmark.
出版信息
Bio Protoc. 2019 Jan 20;9(2):e3146. doi: 10.21769/BioProtoc.3146.
Abstract
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
摘要
胰岛素抵抗是一种由对胰岛素反应受损所定义的病理生理状态，是几种代谢性疾病的危险因素，最显著的是2型糖尿病。胰岛素抵抗发生在包括肝脏、脂肪和骨骼肌在内的胰岛素靶组织中。诸如胰岛素耐量试验和高胰岛素-正常血糖钳夹等方法允许评估特定组织中的胰岛素反应，并有助于研究胰岛素抵抗的进展和原因。在此，我们详细介绍一种在静脉注射胰岛素的麻醉小鼠中评估脂肪和肌肉组织胰岛素作用的方案。
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