Nishida Atsuhiro, Miyawaki Yoshiki, Node Koichi, Akashi Makoto
The Research Institute for Time Studies, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, Yamaguchi, Japan.
Department of Cardiovascular Medicine, Saga University, 5-1-1 Nabeshima, Saga, Japan.
Bio Protoc. 2019 Jan 20;9(2):e3148. doi: 10.21769/BioProtoc.3148.
tissue-culture experiments are often performed in the field of circadian biology. The major aim of these experiments is to evaluate circadian characteristics such as period length at the tissue-autonomous level by monitoring clock gene expression in real time. This culture method is also used to examine the tissue specificity of circadian entrainment factors. However, an culture method for monitoring clock gene expression in hair follicles has yet to be established. In the present study, we developed an experimental method to analogize and evaluate circadian characteristics by performing culture of mouse whisker follicles and monitoring clock gene expression in real time.
组织培养实验常在昼夜节律生物学领域进行。这些实验的主要目的是通过实时监测时钟基因表达，在组织自主水平评估昼夜节律特征，如周期长度。这种培养方法也用于检测昼夜节律同步因子的组织特异性。然而，一种用于监测毛囊中时钟基因表达的培养方法尚未建立。在本研究中，我们开发了一种实验方法，通过对小鼠须毛囊进行培养并实时监测时钟基因表达，来模拟和评估昼夜节律特征。
