利用悬滴法和视黄酸将小鼠胚胎干细胞分化为神经元细胞
Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells Using Hanging Droplets and Retinoic Acid.
作者信息
Witteveldt Jeroen, Macias Sara
机构信息
Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3FL, UK.
出版信息
Bio Protoc. 2019 Nov 5;9(21):e3417. doi: 10.21769/BioProtoc.3417.
Abstract
Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.
摘要
胚胎干细胞的定向分化是干细胞研究中的一项重要工具。在本方案中，我们描述了一种简单的分化方案，该方案涉及使用悬滴法诱导小鼠胚胎干细胞（mESC）形成胚状体，随后分化为神经谱系。
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