一种用于胚胎体形成的基于聚二甲基硅氧烷的微流控悬滴芯片。
A PDMS-Based Microfluidic Hanging Drop Chip for Embryoid Body Formation.
作者信息
Wu Huei-Wen, Hsiao Yi-Hsing, Chen Chih-Chen, Yet Shaw-Fang, Hsu Chia-Hsien
机构信息
Institutes of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Zhunan 35053, Taiwan.
Institute of Nano Engineering and MicroSystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
出版信息
Molecules. 2016 Jul 6;21(7):882. doi: 10.3390/molecules21070882.
The conventional hanging drop technique is the most widely used method for embryoid body (EB) formation. However, this method is labor intensive and limited by the difficulty in exchanging the medium. Here, we report a microfluidic chip-based approach for high-throughput formation of EBs. The device consists of microfluidic channels with 6 × 12 opening wells in PDMS supported by a glass substrate. The PDMS channels were fabricated by replicating polydimethyl-siloxane (PDMS) from SU-8 mold. The droplet formation in the chip was tested with different hydrostatic pressures to obtain optimal operation pressures for the wells with 1000 μm diameter openings. The droplets formed at the opening wells were used to culture mouse embryonic stem cells which could subsequently developed into EBs in the hanging droplets. This device also allows for medium exchange of the hanging droplets making it possible to perform immunochemistry staining and characterize EBs on chip.
传统的悬滴法是用于形成胚状体（EB）的最广泛使用的方法。然而，这种方法劳动强度大，并且受到培养基更换困难的限制。在此，我们报告一种基于微流控芯片的方法，用于高通量形成EB。该装置由在玻璃基板支撑的聚二甲基硅氧烷（PDMS）中具有6×12个开口孔的微流控通道组成。PDMS通道通过从SU-8模具复制聚二甲基硅氧烷（PDMS）制成。用不同的静水压力测试芯片中的液滴形成，以获得直径为1000μm开口孔的最佳操作压力。在开口孔处形成的液滴用于培养小鼠胚胎干细胞，这些细胞随后可在悬滴中发育成EB。该装置还允许对悬滴进行培养基更换，从而有可能在芯片上进行免疫化学染色和表征EB。
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