Jiang Liuliu, Chen Shengyan, Sun Kairui, Zhou Peng, Wei Xin
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
Department of Oral Medicine, Stomatology Hospital Affiliated to Nanjing Medical University, Nanjing, China.
Bio Protoc. 2019 Dec 5;9(23):e3461. doi: 10.21769/BioProtoc.3461.
is the most common cause of fungal infections worldwide. Infection by is closely associated with its ability to form a biofilm, closely packed communities of cells attached to the surfaces of human tissues and implanted devices, in or on the host. When tested for susceptibility to antifungals, such as polyenes, azoles, and allylamines, cells in a biofilm are more resistant to antifungal agents than cells in the planktonic form. Cyclic Adenosine monophosphate (cAMP) is one of the key elements for triggering hyphal and biofilm formation in . It is hard to detect or extract molecular markers (, cAMP) from biofilms because the biofilms have a complex three-dimensional architecture with an extracellular matrix surrounding the cell walls of the cells in the biofilm. Here, we present an improved protocol that can effectively measure the level of intracellular cAMP in biofilms.
是全球范围内真菌感染的最常见原因。由其引起的感染与其形成生物膜的能力密切相关，生物膜是紧密堆积的细胞群落，附着在人体组织表面和植入装置上，存在于宿主体内或体表。当测试对多烯类、唑类和烯丙胺类等抗真菌药物的敏感性时，生物膜中的细胞比浮游形式的细胞对抗真菌剂更具抗性。环磷酸腺苷（cAMP）是触发其菌丝和生物膜形成的关键因素之一。由于生物膜具有复杂的三维结构，其细胞细胞壁周围有细胞外基质，因此很难从生物膜中检测或提取分子标记物（如cAMP）。在此，我们提出了一种改进的方案，该方案可以有效地测量生物膜中细胞内cAMP的水平。
