Kessel D, Thompson P, Musselman B, Chang C K
Cancer Res. 1987 Sep 1;47(17):4642-5.
A procedure involving the use of the reducing agent lithium aluminum hydride (LiAlH4) has been designed to explore the nature of the oligomer linkages in the tumor-localizing component of hematoporphyrin derivative (HPD). High-performance liquid chromatography and fast-atom bombardment mass spectrometry were used to determine the reduction products. The results are consistent with a structure wherein ester linkages join hematoporphyrin molecules. The presence of minor amounts of ether-linked porphyrins was confirmed, and their origin was determined through the application of the chemical reduction process to HPD. An increased proportion of ether-linked porphyrins was detected during storage of HPD at room temperature or above. The commercial product Photofrin II, presumably an HPD preparation enriched in the dimer/oligomer fraction, was found to contain approximately 50% ether linkages. This product therefore differs from the corresponding fraction of freshly prepared HPD.
设计了一种使用还原剂氢化铝锂（LiAlH4）的程序，以探究血卟啉衍生物（HPD）肿瘤定位成分中低聚物键的性质。使用高效液相色谱法和快原子轰击质谱法来确定还原产物。结果与酯键连接血卟啉分子的结构一致。证实了存在少量醚键连接的卟啉，并通过对HPD应用化学还原过程确定了它们的来源。在室温或更高温度下储存HPD期间，检测到醚键连接的卟啉比例增加。商业产品光卟啉II，大概是一种富含二聚体/低聚物部分的HPD制剂，被发现含有约50%的醚键。因此，该产品与新制备的HPD的相应部分不同。
