Weidolf L O, Lee E D, Henion J D
Equine Drug Testing and Toxicology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14850.
Biomed Environ Mass Spectrom. 1988 Mar 1;15(5):283-9. doi: 10.1002/bms.1200150508.
Sulfoconjugated anabolic steroids were separated by micro-bore high-performance liquid chromatography. The eluent was introduced into the atmospheric pressure ion source of the triple-quadrupole mass spectrometer via an ion spray liquid chromatograph/mass spectrometer interface operated in the negative ion mode. The limit of detection was 10 pg on-column by selected ion monitoring of the molecular ion and the response increased linearly over a concentration range of 2.4 orders of magnitude. Following work-up by a liquid-solid extraction procedure of equine urine samples, full-scan daughter ion spectra of boldenone sulfate could be obtained up to 17 days after a therapeutic dose of boldenone undecylenate to a horse.
硫酸共轭合成代谢类固醇通过微径高效液相色谱法进行分离。洗脱液通过在负离子模式下运行的离子喷雾液相色谱/质谱仪接口引入三重四极杆质谱仪的大气压离子源。通过对分子离子进行选择离子监测，柱上检测限为10皮克，并且在2.4个数量级的浓度范围内响应呈线性增加。对马尿样品进行液固萃取程序处理后，在给一匹马治疗剂量的十一酸睾酮后长达17天，均可获得硫酸去甲雄三烯醇酮的全扫描子离子谱。
