针对低微升量哺乳动物血浆的优化氧化应激方案
Optimized Oxidative Stress Protocols for Low-microliter Volumes of Mammalian Plasma.
作者信息
Langille Evan A
机构信息
Department of Chemistry and Biology, Bishop's University, Sherbrooke, Canada.
Department of Chemistry, Memorial University of Newfoundland, St. John's, Canada.
出版信息
Bio Protoc. 2019 May 5;9(9):e3221. doi: 10.21769/BioProtoc.3221.
Small blood volumes commonly obtained from small mammals during field studies are only sufficient for a single biochemical assay. In this study, we used blood collected from a population of wild eastern chipmunks () and developed modified methods to improve analytical selectivity and sensitivity required for measuring markers of oxidative stress using small blood volumes. Specifically, we proposed a modified malondialdehyde (MDA) analysis protocol by high performance liquid chromatography (HPLC) and also optimized both the uric acid independent ferric reducing antioxidant power (FRAP) and hypochlorous acid shock capacity (HASC) assays. We present methods in which a total volume of less than 60 μl of plasma is required to obtain a comprehensive portrait of an individual's oxidative profile.
在野外研究中，从小型哺乳动物身上采集的少量血液通常仅够进行一次生化检测。在本研究中，我们使用从野生东部花栗鼠种群采集的血液，开发了改进方法，以提高使用少量血液测量氧化应激标志物所需的分析选择性和灵敏度。具体而言，我们提出了一种通过高效液相色谱（HPLC）改进的丙二醛（MDA）分析方案，并优化了尿酸独立铁还原抗氧化能力（FRAP）和次氯酸休克能力（HASC）检测方法。我们展示了一些方法，只需不到60微升的血浆总体积就能全面了解个体的氧化状态。
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