Department of Cardiology, University Hospital Split, School of Medicine, University of Split, Croatia.
Molecules. 2011 Aug 17;16(8):7058-68. doi: 10.3390/molecules16087058.
Determination of the relative contribution of uric acid level increases to the total measured antioxidative activity could be very useful for testing antioxidative products and their effect on human health. The aim of this report is to present a simple spectrophotometric method that combines the measurement of total antioxidative capacity of a sample by ferric reducing/antioxidative power (FRAP) assay, with the uricase-reaction (specific elimination of uric acid), in order to establish and correct for the contribution of uric acid in FRAP values. We measured FRAP values, with (uric acid-independent antioxidant capacity, TAC-UA) and without (total antioxidant capacity, TAC) uricase treatment, and expressed it as μmol/L of uric acid equivalents. In such way, it was possible to determine both total and uric acid-independent antioxidant capacity, plasma uric acid (UA, as the difference between TAC and TAC-UA), and the ratio of the uric acid in total antioxidant capacity (UA/TAC).
测定尿酸水平升高对总抗氧化活性的相对贡献可能对测试抗氧化产品及其对人体健康的影响非常有用。本报告的目的是介绍一种简单的分光光度法，该方法将样品的总抗氧化能力的测定（通过铁还原/抗氧化能力（FRAP）测定）与尿酸酶反应（尿酸的特异性消除）相结合，以确定并校正 FRAP 值中尿酸的贡献。我们测量了 FRAP 值，并用（尿酸非依赖性抗氧化能力，TAC-UA）和不用（总抗氧化能力，TAC）尿酸酶处理，并表示为尿酸当量的 μmol/L。通过这种方式，可以确定总抗氧化能力和尿酸非依赖性抗氧化能力、血浆尿酸（UA，作为 TAC 与 TAC-UA 之间的差异）以及尿酸在总抗氧化能力中的比例（UA/TAC）。
