Marine College, Shandong University, Weihai, 264209, People's Republic of China.
Department of Central Lab, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, 264200, People's Republic of China.
Arch Microbiol. 2021 Jul;203(5):2351-2356. doi: 10.1007/s00203-021-02213-w. Epub 2021 Mar 3.
A novel Gram-stain-negative, aerobic, non-flagellated, non-motile, oval-rod-shaped and light pink to light tawny-pigmented bacterial strain (designated 1151) were isolated from marine green algae obtained from the coastal seawater of Weihai, China. Strain 1151 was found to grow at 15-37 °C (optimum, 33 °C), pH 7.0-9.5 (optimum, 7.5-8.5) and in the presence of 1-6% (w/v) NaCl (optimum, 3%). Cells were oxidase-positive and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 1151 was a member of the genus Sulfitobacter and exhibited the hightest sequence similarity to Sulfitobacter indolifex DSM 14862 (96.6%), followed by the sequence similarity to Sulfitobacter aestuarii hydD52 (96.5%) and Sulfitobacter profundi SAORIC-263 (96.5%). The average nucleotide identity and digital DDH values between strain 1151 and Sulfitobacter indolifex DSM 14862 were 69.9% and 20.9%, respectively. The average amino acid identity between strain 1151 and Sulfitobacter pontiacus DSM 10014 (type strain of the type species) was 62.3%. Q-10 was detected as the sole respiratory quinone. The dominant cellular fatty acids were sum feature 8 (Cω7c; 44.1%), Cω7c (29.7%) and C (11.7%). The DNA G + C content of strain 1151 was 51.8 mol%. The polar lipids included phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), and three unidentified lipids (L1, L2 and L3). Based on the phylogenetic and phenotypic characteristics, strain 1151 is considered to represent a novel species of the genus Sulfitobacter, for which the name Sulfitobacter algicola sp. nov. is proposed. The type strain is 1151 (= KCTC 72513 = MCCC 1H00384).
一株新的革兰氏阴性、需氧、无鞭毛、非运动、椭圆形-杆状、浅粉色到浅黄褐色着色的细菌菌株（命名为 1151）从中国威海沿海海水获得的海洋绿藻中分离得到。菌株 1151 被发现可以在 15-37°C（最佳温度 33°C）、pH 值 7.0-9.5（最佳 pH 值 7.5-8.5）和 1-6%（w/v）NaCl（最佳 3%）的存在下生长。细胞氧化酶阳性，过氧化氢酶阳性。基于 16S rRNA 基因序列的系统发育分析表明，菌株 1151 是 Sulfitobacter 属的一个成员，与 Sulfitobacter indolifex DSM 14862 的序列相似度最高（96.6%），其次是与 Sulfitobacter aestuarii hydD52（96.5%）和 Sulfitobacter profundi SAORIC-263（96.5%）的序列相似度。菌株 1151 和 Sulfitobacter indolifex DSM 14862 之间的平均核苷酸同一性和数字 DDH 值分别为 69.9%和 20.9%。菌株 1151 和 Sulfitobacter pontiacus DSM 10014（模式种的模式株）之间的平均氨基酸同一性为 62.3%。检测到 Q-10 作为唯一的呼吸醌。主要的细胞脂肪酸是总和特征 8（Cω7c；44.1%）、Cω7c（29.7%）和 C（11.7%）。菌株 1151 的 DNA G+C 含量为 51.8 mol%。极性脂包括磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）和三种未鉴定的脂（L1、L2 和 L3）。基于系统发育和表型特征，菌株 1151 被认为代表 Sulfitobacter 属的一个新种，提议命名为 Sulfitobacter algicola sp. nov.。模式菌株为 1151（=KCTC 72513=MCCC 1H00384）。
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