Marine College, Shandong University, Weihai 264209, Shandong, PR China.
Life Science and Technology School, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004659.
A facultatively anaerobic bacterium, strain S0837, was isolated from the marine sediment of Jingzi Wharf, Weihai, China. Cells of the novel strain were Gram-stain-negative, non-flagellated, non-gliding, non-pigmented and rod-shaped. Cells were around 0.3-0.5×1.0-1.4 µm in size and often appeared singly. Optimum growth occurred at 33 °C, with 2 % (w/v) NaCl and at pH 7.0-7.5. On the basis of the results of 16S rRNA gene sequences, stain S0837 had the closest relative with KCTC 32183 (98.0 %). Genome sequencing revealed a genome size of 3 785 026 bp, a G+C content of 59.8 mol% and several genes related with sulphur oxidation. The strain shared 98.0 % 16S rRNA sequence similarities with closely related type species and shared ANI value below 95-96 %, dDDH value of showed relatedness of 27.4, 25.2 and 25.2 % respectively with the closely related type species. Strain S0837 had ubiquinone-10 as the sole respiratory quinone, and possessed summed feature 8 (C 7cC 6c) as the major fatty acid. The major polar lipids were phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain S0837 should represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is S0837 (=MCCC 1K04635=KCTC 72860).
一株兼性厌氧菌，菌株 S0837，从中国威海靖子码头的海洋沉积物中分离得到。新菌株的细胞革兰氏阴性，无鞭毛，不滑行，非色素，杆状。细胞大小约为 0.3-0.5×1.0-1.4μm，通常单独出现。最佳生长条件为 33°C、2%（w/v）NaCl 和 pH7.0-7.5。基于 16S rRNA 基因序列的结果，菌株 S0837 与 KCTC 32183（98.0%）最为接近。基因组测序显示其基因组大小为 3785026bp，G+C 含量为 59.8mol%，并且有几个与硫氧化相关的基因。该菌株与密切相关的模式种共享 98.0%的 16S rRNA 序列相似性，与密切相关的模式种的 ANI 值低于 95-96%，dDDH 值分别显示出 27.4%、25.2%和 25.2%的相关性。菌株 S0837 以泛醌-10 作为唯一的呼吸醌，并且拥有 sumFeature 8（C 7cC 6c）作为主要脂肪酸。主要极性脂为磷脂酰甘油、磷脂酰胆碱和磷脂酰乙醇胺。根据表型、化学分类学特征、系统发育特性和基因组分析的结果，菌株 S0837 应代表一个新的属种，建议命名为 sp. nov.。模式菌株为 S0837（=MCCC 1K04635=KCTC 72860）。
