Department of Medical Technology, Kobe Tokiwa University.
Department of Clinical Laboratory, Kobe University Hospital.
Medicine (Baltimore). 2021 Mar 5;100(9):e24556. doi: 10.1097/MD.0000000000024556.
Previous studies from various countries have reported anti-dense fine speckled pattern (DFS)70 antibody prevalence but few studies have been from Asia. We investigated the prevalence of anti-DFS70 autoantibodies in a Japanese cohort of healthy individuals (HI) and patients with antinuclear antibody-associated autoimmune rheumatic diseases (AARD).Enzyme-linked immunosorbent assay and indirect immunofluorescence were performed using samples from 250 HI and 276 AARD patients.The overall anti-DFS70 antibody prevalence in HI was 16.4%, with 12.8% for males and 20.0% for females (sex difference; P = .12). In AARD patients, the anti-DFS70 antibody prevalence in systemic lupus erythematosus, mixed connective tissue disease, systemic sclerosis, dermatomyositis and polymyositis (DM/PM), Sjögren syndrome, and rheumatoid arthritis (RA) was 22.1%, 14.3%, 14.3%, 3.0%, 21.3%, and 18.1%, respectively (no significant difference between AARD patients except DM/PM and HI). The prevalence of isolated anti-DFS70 antibody in HI and all AARD patients excluding RA was 14.8% (37/250) and 4.4% (9/204), respectively (P < .01 vs HI). Among anti-DFS70 antibody-positive cases, 63.4% (26/41) were DFS pattern by IIF and 23.5% (8/34) were HI and AARD patients excluding RA, respectively.The anti-DFS70 antibody prevalence in HI and AARD patients in Japan was similar. Furthermore, the anti-DFS70 antibody prevalence in HI and AARD in Japan is higher than in HI and AARD in regions other than Asia. This makes AARD differential diagnosis by antinuclear antibody screening difficult.
以前来自不同国家的研究报告了抗密集细斑点型（DFS）70 抗体的流行率，但来自亚洲的研究较少。我们调查了日本健康个体（HI）和抗核抗体相关自身免疫性风湿病（AARD）患者中抗-DFS70 自身抗体的流行率。使用来自 250 名 HI 和 276 名 AARD 患者的样本进行酶联免疫吸附测定和间接免疫荧光。HI 中抗-DFS70 抗体的总流行率为 16.4%，男性为 12.8%，女性为 20.0%（性别差异；P=0.12）。在 AARD 患者中，系统性红斑狼疮、混合性结缔组织病、系统性硬化症、皮肌炎和多发性肌炎（DM/PM）、干燥综合征和类风湿关节炎（RA）中抗-DFS70 抗体的流行率分别为 22.1%、14.3%、14.3%、3.0%、21.3%和 18.1%（除 DM/PM 和 HI 外，AARD 患者之间无显著差异）。HI 和除 RA 外的所有 AARD 患者中孤立性抗-DFS70 抗体的流行率分别为 14.8%（37/250）和 4.4%（9/204）（均 P<0.01 与 HI 相比）。在抗-DFS70 抗体阳性病例中，63.4%（26/41）为 IIF 的 DFS 模式，23.5%（8/34）为 HI 和 AARD 患者，除 RA 外。日本 HI 和 AARD 患者的抗-DFS70 抗体流行率相似。此外，日本 HI 和 AARD 中的抗-DFS70 抗体流行率高于亚洲以外地区的 HI 和 AARD。这使得通过抗核抗体筛查进行 AARD 鉴别诊断变得困难。
