Deng Chuiwen, Wang Anqi, Hu Chaojun, Zhang Wen, Zeng Xiaofeng, Fei Yunyun
Department of Rheumatology and Clinical Immunology, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science and Technology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Front Med (Lausanne). 2022 May 10;9:829436. doi: 10.3389/fmed.2022.829436. eCollection 2022.
Although the dense fine speckled (DFS) immunofluorescence staining pattern has been studied by various researchers in recent years, its clinical associations remain unspecified. Thus, we performed a retrospective study in a non-selective population to explore the prevalence of this enigmatic antinuclear antibody (ANA) pattern and to determine its possible clinical associations with any identifiable pathology.
We retrieved the results of ANA testing ordered by various departments in 2019 to study the prevalence of DFS pattern. Demographic characteristics and clinical features of these participants were also collected from the electronic medical record system. Correlation analysis was made to study its clinical associations and a -value < 0.05 was considered statistically significant.
The prevalence of ANA positivity was 37.4% among 72,204 serum samples of which the median age was 44 (interquartile range: 31, 56) years old and 68.0% were women. The prevalence of the DFS staining pattern was 1.1% in the total population and accounted for 3.1% in the ANA-positive population. There were 97.6% of these cases displaying the DFS pattern with a low titer of ANA (≤1:320; starting serum dilution: 1:100). We found that this pattern correlated with several pathological conditions, such as skin disorders (25.1%), alopecia (4.6%), and obstetric complications (6.6%).
The presence of the DFS immunofluorescence staining pattern may accompany several pathological conditions and may be a signal of localized inflammation within certain organs or tissues, especially the skin.
尽管近年来众多研究人员对密集细颗粒状（DFS）免疫荧光染色模式进行了研究，但其临床关联仍不明确。因此，我们在一个非选择性人群中进行了一项回顾性研究，以探究这种神秘的抗核抗体（ANA）模式的患病率，并确定其与任何可识别病理状况之间可能存在的临床关联。
我们检索了2019年各科室进行的ANA检测结果，以研究DFS模式的患病率。还从电子病历系统中收集了这些参与者的人口统计学特征和临床特征。进行相关性分析以研究其临床关联，P值<0.05被认为具有统计学意义。
在72204份血清样本中，ANA阳性率为37.4%，其中位年龄为44岁（四分位间距：31，56岁），女性占68.0%。DFS染色模式在总人群中的患病率为1.1%，在ANA阳性人群中占3.1%。这些病例中有97.6%表现为低滴度ANA（≤1:320；起始血清稀释度：1:100）的DFS模式。我们发现这种模式与多种病理状况相关，如皮肤疾病（25.1%）、脱发（4.6%）和产科并发症（6.6%）。
DFS免疫荧光染色模式的出现可能与多种病理状况相关，可能是某些器官或组织，尤其是皮肤局部炎症的一个信号。
