Regenerative, Modular, and Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway, Galway, Ireland.
Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway, Galway, Ireland.
Am J Physiol Cell Physiol. 2021 May 1;320(5):C842-C849. doi: 10.1152/ajpcell.00380.2020. Epub 2021 Mar 3.
The development of therapeutic regenerative medicine and accurate drug discovery cell-based products requires effective, with respect to obtaining sufficient numbers of viable, proliferative, and functional cell populations, cell expansion ex vivo. Unfortunately, traditional cell culture systems fail to recapitulate the multifaceted tissue milieu in vitro, resulting in cell phenotypic drift, loss of functionality, senescence, and apoptosis. Substrate-, environment-, and media-induced approaches are under intense investigation as a means to maintain cell phenotype and function while in culture. In this context, herein, the potential of macromolecular crowding, a biophysical phenomenon with considerable biological consequences, is discussed.
治疗性再生医学和精准药物发现细胞产品的发展需要有效的方法，以获得足够数量的具有活力、增殖和功能的细胞群体，进行体外细胞扩增。不幸的是，传统的细胞培养系统无法在体外再现多方面的组织环境，导致细胞表型漂移、功能丧失、衰老和凋亡。目前正在深入研究基质、环境和培养基诱导的方法，以在培养过程中维持细胞表型和功能。在这种情况下，本文讨论了大分子拥挤这一具有重要生物学意义的生物物理现象的潜力。
