Malamed S, Gibney J A, Scanes C G
Department of Anatomy, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
Cell Tissue Res. 1988 Mar;251(3):581-5. doi: 10.1007/BF00214006.
The somatotrophs of the pituitary gland of the male domestic fowl were identified by means of an immunoelectron-microscopic method based on gold as the electron-opaque label and an antibody to growth hormone. Gold particles indicating sites of growth hormone were restricted to cells in which virtually all of the granules were labelled. Little, if any, gold label was found outside the granules in these cells designated as somatotrophs, or at sites outside these cells. The size of these gold-labelled secretory granules presumed to contain growth hormone decreased with age, from a mean sectional diameter of 256 +/- 6.2 nm (SEM) at 4-6 weeks to 221 +/- 5.7 nm at 11-18 weeks and 205 +/- 8.6 nm at 24-30 weeks of age. On the basis of these values for mean sectional diameters the change between the first two periods represents a decrease in granule volume of about 36%. However, during the same period the growth hormone concentration of the granules increased. Accordingly, growth hormone content per granule changed little if at all. In contrast, from 11-18 weeks to 24-30 weeks of age there was a decrease of 31% in growth hormone content per granule. These data indicate that growth hormone packaging in the chicken somatotroph changes with age. The first change results in the production of smaller granules of higher growth hormone concentration. During this period growth hormone content per granule remains relatively constant. The later change results in the production of granules of lower growth hormone content than that of younger animals.
采用以金作为电子不透明标记物和生长激素抗体的免疫电子显微镜方法，对雄性家禽脑垂体的促生长激素细胞进行了鉴定。指示生长激素位点的金颗粒仅限于几乎所有颗粒都被标记的细胞。在这些被指定为促生长激素细胞的细胞中，颗粒外几乎没有发现金标记，在这些细胞外的位点也未发现金标记。这些推测含有生长激素的金标记分泌颗粒的大小随年龄增长而减小，4至6周龄时平均截面直径为256±6.2纳米（标准误），11至18周龄时为221±5.7纳米，24至30周龄时为205±8.6纳米。根据这些平均截面直径的值，前两个时期之间的变化表示颗粒体积减少了约36%。然而，在同一时期，颗粒中的生长激素浓度增加。因此，每个颗粒的生长激素含量几乎没有变化。相比之下，从11至18周龄到24至30周龄，每个颗粒的生长激素含量下降了31%。这些数据表明，鸡促生长激素细胞中生长激素的包装随年龄而变化。第一个变化导致产生生长激素浓度更高的较小颗粒。在此期间，每个颗粒的生长激素含量保持相对恒定。后期变化导致产生的颗粒生长激素含量低于年轻动物。
