Department of Crop Animal Production, Avanos Vocational School, University of Nevşehir Hacı Bektaş Veli, Nevşehir, Turkey.
Department of Plant and Animal Production, Vocational School of Technical Sciences, University of Kırşehir Ahi Evran, Kırşehir, Turkey.
Drug Chem Toxicol. 2022 Jul;45(4):1870-1880. doi: 10.1080/01480545.2021.1892948. Epub 2021 Mar 3.
In this study, 38 nm-sized and flake-like-shaped CuO NPs (10, 50, 100, 150 μg/10 µl/larva) were force-fed to fourth instar (100 ± 20 mg) (Lepidoptera: Pyralidae) larvae under the laboratory conditions. The effects of CuO NPs on total hemocyte counts (THCs) and the frequency of viable, mitotic, apoptotic, necrotic, and micronucleated hemocyte indices were detected with the double-staining protocol by hematoxylin and eosin (H&E) stains. The total hemocyte counts (THCs) did not change significantly in . larvae at all concentrations for 24 h and 72 h post-force-feeding treatment. The ratio of viable hemocytes decreased at 50, 100, 150 μg/10 µl concentrations in 24 h and 72 h when compared with untreated larvae. The increases in the percentage of mitotic and micronucleated hemocytes were statistically significant at 150 μg/10 µl in 24 h. The results showed that high concentrations (>10 μg/10 µl) of CuO NPs increased the percentage of apoptotic hemocytes in 24 h. 100 and 150 μg/10 µl of CuO NPs caused a significant increase in the percentage of necrotic hemocytes in 24 h. The decrease in the percentage of mitotic hemocytes at 10, 100 and 150 μg/10 µl in 72 h was statistically significant. Apoptotic hemocytes increased and were found to be higher at 100 and 150 μg/10 µl of CuO NPs in 72 h in comparison with the untreated larvae. Finally, we observed an increase in the percentage of necrotic hemocytes at 150 μg/10 µl in 72 h.
在这项研究中，将 38nm 大小和片状形状的氧化铜纳米颗粒（10、50、100、150μg/10μl/幼虫）通过强制喂食的方式给第四龄期（100±20mg）（鳞翅目：Pyralidae）幼虫，在实验室条件下进行。通过苏木精和曙红（H&E）染色的双重染色方案检测氧化铜纳米颗粒对总血细胞计数（THCs）和活细胞、有丝分裂、凋亡、坏死和微核血细胞指数的频率的影响。在所有浓度下，在 24 小时和 72 小时的强制喂食处理后，幼虫的总血细胞计数（THCs）没有显著变化。与未处理的幼虫相比，在 24 小时和 72 小时时，在 50、100、150μg/10μl 浓度下活细胞的比例降低。在 24 小时时，有丝分裂和微核血细胞的百分比增加具有统计学意义，在 150μg/10μl 时达到最高。结果表明，高浓度（>10μg/10μl）的氧化铜纳米颗粒增加了 24 小时时凋亡血细胞的百分比。在 24 小时时，100 和 150μg/10μl 的氧化铜纳米颗粒导致坏死血细胞的百分比显著增加。在 72 小时时，在 10、100 和 150μg/10μl 时有丝分裂血细胞的百分比减少具有统计学意义。在 72 小时时，与未处理的幼虫相比，凋亡血细胞增加，在 100 和 150μg/10μl 的氧化铜纳米颗粒时更高。最后，我们观察到在 72 小时时，在 150μg/10μl 时坏死血细胞的百分比增加。
