Department of Bioengineering, Faculty of Engineering, Adana Alparslan Türkeş Science and Technology University, Adana, Turkey.
Department of Veterinary Medicine, Çaycuma Food and Agriculture Vocational School, Zonguldak Bülent Ecevit University, Zonguldak, Turkey.
Bull Environ Contam Toxicol. 2021 Sep;107(3):412-420. doi: 10.1007/s00128-021-03261-0. Epub 2021 May 17.
In this study, the effects of dietary CuO nanoparticles (NPs) on metabolic enzyme activity, biochemical parameters, and total (THC) and differential hemocyte counts (DHC) were determined in Galleria mellonella larvae. Using concentrations of 10, 100, 1000 mg/L and the LC10 and LC30 levels of CuO NPs, we determined that the NPs negatively impacted metabolic enzyme activity and biochemical parameters in larval hemolymph. Compared with the control, the greatest increase in THC was observed in larvae fed on diets with 100 mg L of CuO NPs. Plasmatocytes and granulocytes were among the most numerous hemocytes in all treatments. These results suggest that dietary CuO NPs effects the metabolic metabolism and immune system of G. mellonella and provide indirect information regarding the toxic effects of CuO NPs in mammalian immune system given similarities between mammalian blood cells and insect hemocytes.
在这项研究中，我们研究了膳食氧化铜纳米颗粒（NPs）对欧洲山蜂幼虫代谢酶活性、生化参数以及总（THC）和差异血细胞计数（DHC）的影响。我们使用了 10、100、1000 mg/L 的浓度以及 CuO NPs 的 LC10 和 LC30 水平，结果表明 NPs 对幼虫血淋巴中的代谢酶活性和生化参数有负面影响。与对照组相比，在喂食含 100 mg/L CuO NPs 的饮食的幼虫中，THC 增加最多。在所有处理中，浆细胞和粒细胞都是数量最多的血细胞。这些结果表明，膳食氧化铜纳米颗粒影响欧洲山蜂幼虫的新陈代谢和免疫系统，并为氧化铜纳米颗粒在哺乳动物免疫系统中的毒性效应提供了间接信息，因为哺乳动物血细胞和昆虫血细胞之间存在相似性。
