Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.
Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
J Clin Microbiol. 2021 Apr 20;59(5). doi: 10.1128/JCM.00089-21.
Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for detection. Of the 379 concordant -positive samples, 86.8% were found to possess the S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.
耐药指导治疗（RGT）淋病可能会减少广谱抗生素的不必要使用。当从 Aptima Combo 2 检测中反射时，ResistancePlus GC 检测对检测的敏感性为 94.8%，特异性为 100.0%。在 379 个一致阳性样本中，86.8%发现存在 S91F 突变，该突变对环丙沙星耐药性具有高度预测性，并且在 3144 个公开可用的基因组中稳定。我们的工作支持将淋病的 RGT 纳入常规分子工作流程的可行性。
