Carew J A, Collins M F, Kennedy A R
Laboratory of Radiobiology, Harvard School of Public Health, Boston, MA 02115.
Carcinogenesis. 1988 May;9(5):725-9. doi: 10.1093/carcin/9.5.725.
Bacterial endotoxins (lipopolysaccharides) were found to suppress X-ray-induced malignant transformation of C3H/10T1/2 cells. Endotoxins were effective if present either throughout the 6-week transformation assay period, or for the final 4-week phase, but not when present only for the initial 2-week phase. Neither growth nor survival of C3H/10T1/2 cells, or a radiation-transformed cell line derived from them, were affected by endotoxins. Also, the endotoxins did not affect the formation of foci by the radiation transformed cells when these cells were co-cultured with untransformed cells. These results suggest that endotoxins exert their effect directly upon the transformation process itself, perhaps at a 'late' step in the conversion of an untransformed to a transformed cell.
研究发现细菌内毒素（脂多糖）可抑制X射线诱导的C3H/10T1/2细胞恶性转化。若内毒素在整个6周的转化试验期内存在，或在最后4周阶段存在，则具有抑制作用，但仅在最初2周阶段存在时则无此作用。内毒素对C3H/10T1/2细胞及其衍生的辐射转化细胞系的生长和存活均无影响。此外，当辐射转化细胞与未转化细胞共培养时，内毒素也不影响辐射转化细胞形成集落。这些结果表明，内毒素可能在未转化细胞向转化细胞转变的“后期”步骤，直接对转化过程本身发挥作用。
