实时荧光定量PCR检测方法在检测实验感染鸡肝脏中猪蛔虫DNA的应用
Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens.
作者信息
Nguyen Yen Thi Hoang, Nonaka Nariaki, Maruyama Haruhiko, Yoshida Ayako
机构信息
Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai Nishi, Miyazaki 889-2192, Japan.
Department of Parasitology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Trau Quy, Gia Lam, Ha Noi 100000, Vietnam.
出版信息
J Vet Med Sci. 2021 Apr 9;83(4):671-674. doi: 10.1292/jvms.20-0404. Epub 2021 Mar 4.
This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.
本研究旨在评估使用实时荧光定量PCR检测鸡肝脏中猪蛔虫幼虫DNA的采样方法。给鸡接种单剂量猪蛔虫卵（A组）或多次低剂量猪蛔虫卵（B组）。在B组最后一次感染后第3天和A组第14天开始持续观察肝脏上的白斑（WSs）。在A组中，WS病变处检测到幼虫DNA的比例（78.6%）显著高于其余组织样本（31.3%）。总之，将WS病变应用于检测提高了鸡肝脏中猪蛔虫DNA的检出率，尤其是在单次感染的情况下。
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